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	Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w])
	Li, Meina; Xu, Wenying; Yang, Wenqiang; Kong, Zhaosheng; Xue, Yongbiao
刊名	Plant physiology
	2007-08-01
卷号	144期号:4页码:1797-1812
ISSN号	0032-0889
通讯作者	Xue, yongbiao(ybxue@genetics.ac.cn)
英文摘要	In angiosperms, the stigma provides initial nutrients and guidance cues for pollen grain germination and tube growth. however, little is known about the genes that regulate these processes in rice (oryza sativa). here, we generate rice stigma-specific or -preferential gene expression profiles through comparing genome-wide expression patterns of hand-dissected, unpollinated stigma at anthesis with seven tissues, including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds 5 d after pollination, 10-d-old embryo, 10-d-old endosperm, and suspension-cultured cells by using both 57 k affymetrix rice whole-genome array and 10 k rice cdna microarray. a high reproducibility of the microarray results was detected between the two different technology platforms. in total, we identified 548 genes to be expressed specifically or predominantly in the stigma papillar cells of rice. real-time quantitative reverse transcription-polymerase chain reaction analysis of 34 selected genes all confirmed their stigma-specific expression. the expression of five selected genes was further validated by rna in situ hybridization. gene ontology analysis shows that several auxin-signaling components, transcription, and stress-related genes are significantly overrepresented in the rice stigma gene set. interestingly, most of them also share several cisregulatory elements with known stress-responsive genes, supporting the notion of an overlap of genetic programs regulating pollination and stress/defense responses. we also found that genes involved in cell wall metabolism and cellular communication appear to be conserved in the stigma between rice and arabidopsis (arabidopsis thaliana). our results indicate that the stigmas appear to have conserved and novel molecular functions between rice and
WOS关键词	RICE ORYZA-SATIVA ; POLLEN-TUBE GROWTH ; SELF-INCOMPATIBILITY ; CELL-WALL ; S-LOCUS ; SEXUAL REPRODUCTION ; MICROARRAY ANALYSIS ; PISTIL INTERACTIONS ; FLOWER DEVELOPMENT ; STRESS RESPONSES
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000248704800010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2381069
专题	中国科学院大学
通讯作者	Xue, Yongbiao
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Mol & Dev Biol Lab, Beijing 100080, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Meina,Xu, Wenying,Yang, Wenqiang,et al. Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w])[J]. Plant physiology,2007,144(4):1797-1812.
APA	Li, Meina,Xu, Wenying,Yang, Wenqiang,Kong, Zhaosheng,&Xue, Yongbiao.(2007).Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w]).Plant physiology,144(4),1797-1812.
MLA	Li, Meina,et al."Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w])".Plant physiology 144.4(2007):1797-1812.

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