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	Regulation of intracellular mek1/2 translocation in mouse oocytes - cytoplasmic dynein/dynactin-mediated poleward transport and cyclin b degradation-dependent release from spindle poles
	Xiong, Bo; Yu, Ling-Zhu; Wang, Qiang; Ai, Jun-Shu; Yin, Shen; Liu, Jing-He; OuYang, Ying-Chun; Hou, Yi; Chen, Da-Yuan; Zou, Hui
刊名	Cell cycle
	2007-06-15
卷号	6期号:12页码:1522-1528
关键词	Mek1/2 Dynein/dynactin complex Spindle Pole Cyclin b (delta 90) Mouse oocyte
ISSN号	1538-4101
通讯作者	Sun, qing-yuan(sunqy@ioz.ac.cn)
英文摘要	We recently reported that mek1/2 plays an important role in microtubule organization and spindle pole tethering in mouse oocytes, but how the intracellular transport of this protein is regulated remains unknown. in the present study, we investigated the mechanisms of poleward mek1/2 transport during the prometaphase i/metaphase i transition and mek1/2 release from the spindle poles during the metaphase i/anaphase i transition in mouse oocytes. firstly, we found that p-mek1/2 was colocalized with dynactin at the spindle poles. inhibition of the cytoplasmic dynein/dynactin complex by antibody microinjection blocked polar accumulation of p-mek1/2 and caused obvious spindle abnormalities. moreover, coimmunoprecipitation of p-mek1/2 and dynein or dynactin from mouse oocyte extracts confirmed their association at metaphase i. secondly, disruption of microtubules by nocodazole resulted in the failure of poleward p-mek1/2 transport. whereas, when the nocodazole-treated oocytes were recovered in fresh culture medium, the spindle reformed and p-mek1/2 relocalized to the spindle poles. finally, we examined the mechanism of p-mek1/2 release from the spindle poles. in control oocytes, polar p- mek1/2 was gradually released during metaphase i/anaphase i transition. by contrast, in the presence of nondegradable cyclin b (delta 90), p-mek1/2 still remained at the spindle poles at anaphase i. our results indicate that poleward mek1/2 transport is a cytoplasmic dynein/dynactin-mediated and spindle microtubule-dependent intracellular movement, and that its subsequent anaphase release from spindle poles is dependent on cyclin b degradation.
WOS关键词	ACTIVATED PROTEIN-KINASE ; MATURATION-PROMOTING FACTOR ; MAP KINASE ; MEIOTIC MATURATION ; PIG OOCYTES ; CHECKPOINT INACTIVATION ; XENOPUS OOCYTES ; GAMMA-TUBULIN ; CELL-DIVISION ; MEIOSIS I
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	LANDES BIOSCIENCE
WOS记录号	WOS:000249090900020
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380730
专题	中国科学院大学
通讯作者	Sun, Qing-Yuan
作者单位	1.Chinese Acad Sci, State Key Lab Reprod Biol, Inst Zool, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China 3.Univ Texas, SW Med Ctr, Dept Biol Mol, Dallas, TX USA
推荐引用方式 GB/T 7714	Xiong, Bo,Yu, Ling-Zhu,Wang, Qiang,et al. Regulation of intracellular mek1/2 translocation in mouse oocytes - cytoplasmic dynein/dynactin-mediated poleward transport and cyclin b degradation-dependent release from spindle poles[J]. Cell cycle,2007,6(12):1522-1528.
APA	Xiong, Bo.,Yu, Ling-Zhu.,Wang, Qiang.,Ai, Jun-Shu.,Yin, Shen.,...&Sun, Qing-Yuan.(2007).Regulation of intracellular mek1/2 translocation in mouse oocytes - cytoplasmic dynein/dynactin-mediated poleward transport and cyclin b degradation-dependent release from spindle poles.Cell cycle,6(12),1522-1528.
MLA	Xiong, Bo,et al."Regulation of intracellular mek1/2 translocation in mouse oocytes - cytoplasmic dynein/dynactin-mediated poleward transport and cyclin b degradation-dependent release from spindle poles".Cell cycle 6.12(2007):1522-1528.

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