QQ客服 官方微博 反馈留言

	Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression
	Cai, Hancheng; Yin, Duanzhi; Zhang, Lan; Yang, Xlaofeng; Xu, Xiaoyan; Liu, Weiguo; Zheng, Xuesheng; Zhang, Hong; Wang, Jing; Xu, Yuhong
刊名	Nuclear medicine and biology
	2007-08-01
卷号	34期号:6页码:717-725
关键词	6-[f-18]fpcv Hsv1-tk Gene expression Pet
ISSN号	0969-8051
DOI	10.1016/j.nucmedbio.2007.06.005
通讯作者	Yin, duanzhi(chcbati@yahoo.com.cn)
英文摘要	Introduction: 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) was prepared via a one-step nucleophilic substitution and evaluated as a novel probe for imaging the expression of herpes simplex virus type i thymidine kinase (hsvi-tk) reporter gene. methods: log p of 6-[18f]fpcv was calculated in octanol/phosphate-buffered saline (pbs). stability studies were performed in pbs and bovine serum albumin (bsa). cell uptake was performed at various time points in wild-type cells and transduced cells. for in vivo studies, tumors were grown in nude mice by inoculation with c6 cells, wild type and tk positive. the radiotracer was intravenously injected to animals, and micro-pet imaging was performed. biodistribution of 6-[f-18]fpcv was performed on another group of animals at different time points. results: log p of 6-[f-18]fpcv was -0.517. 6-[f-18]fpcv was fairly stable in pbs and bsa at 6 h. the tracer uptake in c6-tk cells was 5.5-18.8 times higher than that in wild-type cells, the plasma half-life of 6-[f-18]fpcv was as follows: u t(1/2)=1.2 min and 1 t(1/2) = 73.7 min. the average ratio of tumor uptake between the transduced tumor and the wild-type tumor was 1.69 at 15 min. conclusion: biological evaluation showed that 6-[18f]fpcv is a potential probe for imaging hsv1 -tk gene expression. however, its in vivo defluorination may limit its application in pet imaging of gene expression. (c) 2007 elsevier inc. all rights reserved.
WOS关键词	POSITRON-EMISSION-TOMOGRAPHY ; IN-VIVO ; THYMIDINE KINASE ; PYRIMIDINE NUCLEOSIDES ; RADIOTRACER ; THERAPY ; ANALOGS ; CANCER ; VITRO
WOS研究方向	Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000249157300016
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380472
专题	中国科学院大学
通讯作者	Yin, Duanzhi
作者单位	1.Chinese Acad Sci, Shanghai Inst Appl Phys, Res Ctr Radiopharmaceut, Shanghai 201800, Peoples R China 2.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Inst Brain Med Sci, Hangzhou 310009, Peoples R China 3.Zhejiang Univ, Med Sch, Hangzhou 310009, Peoples R China 4.Zhejiang Calif Int NanoSyst Inst, Hangzhou 310029, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cai, Hancheng,Yin, Duanzhi,Zhang, Lan,et al. Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression[J]. Nuclear medicine and biology,2007,34(6):717-725.
APA	Cai, Hancheng.,Yin, Duanzhi.,Zhang, Lan.,Yang, Xlaofeng.,Xu, Xiaoyan.,...&Wang, Yongxian.(2007).Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression.Nuclear medicine and biology,34(6),717-725.
MLA	Cai, Hancheng,et al."Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression".Nuclear medicine and biology 34.6(2007):717-725.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。