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	Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection
	Zhou, Huanbin; Li, Shaofang; Deng, Zhiyong; Wang, Xianping; Chen, Tao; Zhang, Jinsong; Chen, Shouyi; Ling, Hongqing; Zhang, Aimin; Wang, Daowen
刊名	Plant journal
	2007-11-01
卷号	52期号:3页码:420-434
关键词	Receptor-like kinase Stripe rust Virus-induced gene silencing Barley stripe mosaic virus Wheat
ISSN号	0960-7412
DOI	10.1111/j.1365-313x.2007.03246.x
通讯作者	Wang, daowen(dwwang@genetics.ac.cn)
英文摘要	Considerable progress has been made in understanding the function of receptor-like kinase (rlk) genes in model plants. however, much less is known about these genes in crop species. here we report the characterization of three new wheat rlk genes (tarlk-r1, 2 and 3). the primary structure of the putative proteins tarlk-r1, 2 and 3 contained a signal peptide, a cysteine-rich extracellular domain, a transmembrane domain, and a predicted intracellular kinase domain. the fusions between tarlk-r1, 2 or 3 and the green fluorescence protein (gfp) were targeted to the plasma membrane; such targeting required the signal peptide, extracellular domain and transmembrane domain. transcription of tarlk-r1, 2 and 3 was found mainly in the green organs, and was regulated by light. transcript levels of tarlk-r1, 2 and 3 increased during the hypersensitive reaction (hr) to stripe rust fungus. in addition, the tarlk-r3 transcript level was also upregulated by abiotic stresses. further experiments revealed that the recombinant kinase domain of tarlk-r3 exhibited auto-phosphorylation activity in vitro. knocking down the transcript levels of tarlk-r1, 2 or 3 individually or all together by virus-induced gene silencing compromised the wheat hr to stripe rust fungus. the demonstration of tarlk-r1, 2 and 3 as positive contributors in the wheat hr to stripe rust fungus suggests a new direction for further functional studies of this important family of rlk genes, and may facilitate the breeding of wheat varieties resistant to stripe rust disease.
WOS关键词	TRITICUM-AESTIVUM L. ; PROTEIN-KINASE ; DISEASE RESISTANCE ; ARABIDOPSIS-THALIANA ; ABSCISIC-ACID ; BARLEY ; FAMILY ; PLANT ; RICE ; ERECTA
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL PUBLISHING, INC
WOS记录号	WOS:000250298600003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380425
专题	中国科学院大学
通讯作者	Wang, Daowen
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Huanbin,Li, Shaofang,Deng, Zhiyong,et al. Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection[J]. Plant journal,2007,52(3):420-434.
APA	Zhou, Huanbin.,Li, Shaofang.,Deng, Zhiyong.,Wang, Xianping.,Chen, Tao.,...&Zhang, Xiangqi.(2007).Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection.Plant journal,52(3),420-434.
MLA	Zhou, Huanbin,et al."Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection".Plant journal 52.3(2007):420-434.

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