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	Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail xiphophorus helleri
	Yang, HP; Hazelwood, L; Walter, RB; Tiersch, TR
刊名	Cryobiology
	2006-04-01
卷号	52期号:2页码:209-218
关键词	Sperm cryopreservation Osmotic pressure Green swordtail xiphophorus helleri
ISSN号	0011-2240
DOI	10.1016/j.cryobiol.2005.11.002
通讯作者	Tiersch, tr()
英文摘要	In this study, refrigerated storage and cryopreservation of sperm from the green swordtail xiphophorus helleri were investigated. previous cryopreservation research in this species utilized motile sperm because unlike in most fish species, xiphophorus sperm can remain continuously motile after collection for it week with refrigerated storage. however, this species reproduces by internal fertilization, and given the significant requirements for motility within the female reproductive tract and potential limitations on sperm energetic capacities, immobilization of sperm prior to insemination could be used to improve fertilization success. thus, the goal in this study was to use osmotic pressure to inhibit the motility of sperm after collection from x. helleri, and to test the effect of immobilization on refrigerated storage and cryopreservation. the objectives were to: (1) estimate the motility of sperm at different osmotic pressures, and determine an osmotic pressure suitable for immobilization; (2) cryopreserve the immobilized sperm, and estimate the motility after thawing with or without dilution, and (3) compare motility of non-immobilized and immobilized sperm after thawing, centrifugation., and washing to remove cryoprotectant. motility was determined when sperm were suspended in 11 different osmotic pressures (24-500 mosmol/kg) of hanks' balanced salt solution (hbss). motility was observed between 116 and 425 mosmol/kg. sperm were not motile when the osmolality was lower than 116 or higher than 425 mosmol/kg. motility of the immobilized (nonmotile) sperm could be activated by changing the osmotic pressure to 291-316 mosmol/kg, and motility of immobilized sperm from hypertonic hbss (>= 425mosmol/kg) was significantly higher than that from hypotonic hbss (<= 145 mosmol/kg) after 48 h of storage. at an osinolality of 500 mosmol/kg, hbss was used as extender to maintain immobilized sperm during cryopreservation with glycerol as the cryoprotectant. high motility (similar to 55%) was obtained in sperm after thawing when cryopreserved with 10-15% glycerol, and dilution of thawed sperm in fresh hbss (1:4; v:v) was found to decrease the motility significantly. no difference was found in the motility of thawed sperm cryopreserved with 14% glycerol and extended in 3 10 and 500 mosmol/kg hbss. washing by centrifugation prolonged the motility of thawed sperm from 24 to 72h in hbss at 310 and 500 mosmol/kg. this study showed that sperm from x. helleri could be immobilized by use of specific osmotic pressures, and that the immobilization did not affect sperm motility after thawing. the immobilization of sperm by osmotic pressure could minimize reduction of the energetic capacities necessary for insemination, traversal, and residence within the female reproductive tract, and fertilization. (c) 2005 elsevier inc. all rights reserved.
WOS关键词	LIVE-BEARING FISH ; HUMAN SPERMATOZOA ; MOTILITY ; OSMOLALITY ; POTASSIUM ; PLATYFISH ; POECILIIDAE ; PHYSIOLOGY ; MACULATUS ; ZEBRAFISH
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Physiology
WOS类目	Biology ; Physiology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000236605300005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379000
专题	中国科学院大学
通讯作者	Tiersch, TR
作者单位	1.Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Aquaculture Res Stn, Baton Rouge, LA 70820 USA 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Texas State Univ, Dept Chem & Biochem, San Marcos, TX 78666 USA
推荐引用方式 GB/T 7714	Yang, HP,Hazelwood, L,Walter, RB,et al. Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail xiphophorus helleri[J]. Cryobiology,2006,52(2):209-218.
APA	Yang, HP,Hazelwood, L,Walter, RB,&Tiersch, TR.(2006).Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail xiphophorus helleri.Cryobiology,52(2),209-218.
MLA	Yang, HP,et al."Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail xiphophorus helleri".Cryobiology 52.2(2006):209-218.

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