| A bac clone-based physical map of ovine major histocompatibility complex | |
| Liu, HB; Liu, K; Wang, JF; Ma, RLZ | |
| 刊名 | Genomics
 |
| 2006-07-01 | |
| 卷号 | 88期号:1页码:88-95 |
| 关键词 | Bacterial artificial chromosome Major histocompatibility complex Ovine leukocyte antigen Physical mapping Sheep |
| ISSN号 | 0888-7543 |
| DOI | 10.1016/j.ygeno.2006.02.006 |
| 通讯作者 | Ma, rlz() |
| 英文摘要 | An ovine bacterial artificial chromosome (bac) library containing 190,000 bac clones was constructed and subsequently screened to construct a bac-based physical map for the ovine major histocompatibility complex (mhc). two hundred thirty-three bac clones were selected by 84 overgo probes designed on human, mouse, and swine mhc sequence homologies. ninety-four clones were ordered by dna fingerprinting to form contigs i, ii, and iii that correspond to ovine mhc class i-class iii, class iia, and class iib. the minimum tiling paths of contigs i, ii, and iii are 15, 4, and 4 bac clones, spanning approximately 1900, 400, and 300 kb, respectively. the order and orientation of most bac clones in each contig were confirmed by bac-end sequencing. an open gap exists between class iia and class iii. this work helps to provide a foundation for detailed study of ovine mhc genes and of evolution of mhcs in mammals. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | CLASS-I GENES ; ARTIFICIAL CHROMOSOME LIBRARY ; GENOMIC SEQUENCE ; MHC ; REGION ; SHEEP ; ORGANIZATION ; CONSTRUCTION ; ALLELES ; LOCALIZATION |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000238552300009 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2378818 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma, RLZ |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, HB,Liu, K,Wang, JF,et al. A bac clone-based physical map of ovine major histocompatibility complex[J]. Genomics,2006,88(1):88-95. |
| APA | Liu, HB,Liu, K,Wang, JF,&Ma, RLZ.(2006).A bac clone-based physical map of ovine major histocompatibility complex.Genomics,88(1),88-95. |
| MLA | Liu, HB,et al."A bac clone-based physical map of ovine major histocompatibility complex".Genomics 88.1(2006):88-95. |
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