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	Transgenic expression of dwmyb2 impairs iron transport from root to shoot in arabidopsis thaliana
	Chen, Yan-Hong; Wu, Xue-Min; Ling, Hong-Qing; Yang, Wei-Cai
刊名	Cell research
	2006-10-01
卷号	16期号:10页码:830-840
关键词	Dwmyb2 Iron uptake and homeostasis Iron transporters Arabidopsis
ISSN号	1001-0602
DOI	10.1038/sj.cr.7310099
通讯作者	Yang, wei-cai(wcyang@genetics.ac.cn)
英文摘要	In plant, iron uptake and homeostasis are tightly regulated to ensure its absorption from soil and to avoid excess iron in the cell. many genes involved in this process have been identified during past several years, but there are many problems remain unsolved in the genetic regulation of whole plant iron trafficking and allocation. myb transcription factors contain tandem repeats of a similar to 50 amino acid dna-binding motif (r) and are involved in the regulation of many aspects of plant development, hormone signaling and metabolism. here, we report that the ectopic expression of orchid r2r3-myb gene dwmyb2 in arabidopsis thaliana confers the transgenic plants hypersensitivity to iron deficiency. in dwmyb2 transgenic plants, the iron content in root is two-fold higher compared to that in wild-type root, while the reverse is true in shoot. this imbalance of iron content in root and shoot suggested that the translocation of iron from root to shoot was affected by the expression of dwmyb2 in the transgenic plants. consistently, gene chip and reverse transcription-polymerase chain reaction analysis revealed that the ferric-chelate reductase gene, atfro2, and the iron transporter gene, atirt1 and atirt2, are up-regulated by dwmyb2 expression, while other potential iron transporters such as atireg1, atfrd3 and nramp1 are down-regulated. in addition, the expression of several putative peptide transporters and transcription factors are also altered in the 35s::dwmyb2 transgenic lines. these data provide us insight into the whole plant translocation of iron and identify candidate genes for iron homeostasis in plants despite the fact that a heterologous gene was expressed.
WOS关键词	FERRIC-CHELATE REDUCTASE ; METAL TRANSPORTER ; GENE FAMILY ; PLANTS ; TOMATO ; DEFICIENCY ; IRT1 ; NICOTIANAMINE ; ACQUISITION ; HOMEOSTASIS
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000242264300005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378614
专题	中国科学院大学
通讯作者	Yang, Wei-Cai
作者单位	1.Chinese Acad Sci, Key Lab Mol & Dev Biol, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Nantong Univ, Sch Life Sci, Nantong 226007, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yan-Hong,Wu, Xue-Min,Ling, Hong-Qing,et al. Transgenic expression of dwmyb2 impairs iron transport from root to shoot in arabidopsis thaliana[J]. Cell research,2006,16(10):830-840.
APA	Chen, Yan-Hong,Wu, Xue-Min,Ling, Hong-Qing,&Yang, Wei-Cai.(2006).Transgenic expression of dwmyb2 impairs iron transport from root to shoot in arabidopsis thaliana.Cell research,16(10),830-840.
MLA	Chen, Yan-Hong,et al."Transgenic expression of dwmyb2 impairs iron transport from root to shoot in arabidopsis thaliana".Cell research 16.10(2006):830-840.

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