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	Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+
	Yi, Y. P.; Wang, X.; Zhang, G.; Fu, T. -S.; Zhang, G. -J.
刊名	General physiology and biophysics
	2006-06-01
卷号	25期号:2页码:149-160
关键词	Lysosome Phosphatidic acid Potassium ion Proton Osmotic sensitivity
ISSN号	0231-5882
通讯作者	Zhang, g. -j.(zhanggj@sun5.ibp.ac.cn)
英文摘要	Lysosomal destabilization is a critical event not only for the organelle but also for living cells. however, what factors can affect lysosomal stability is not fully studied. in this work, the effects of phosphatidic acid (pa) on the lysosomal integrity were investigated. through the measurements of lysosomal beta-hexosaminidase free activity, intralysosomal ph, leakage of lysosomal protons and lysosomal latency loss in hypotonic sucrose medium, we established that pa could increase the lysosomal permeability to k+ and h+, and enhance the lysosomal osmotic sensitivity. treatment of lysosomes with pa promoted entry of k+ into the organelle via k+/h+ exchange, which could produce osmotic stresses and osmotically destabilize the lysosomes. in addition, pa-induced increase in the lysosomal osmotic sensitivity caused the lysosomes to become more liable to destabilization in osmotic shocks. the results suggest that pa may play a role in the lysosomal destabilization.
WOS关键词	PHOSPHOLIPASE-D ; LYSOPHOSPHATIDIC ACID ; ENZYME RELEASE ; PROTON PUMP ; RAT-LIVER ; MEMBRANE ; TRANSLOCATION ; PATHOGENESIS ; ENHANCEMENT ; INVOLVEMENT
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Physiology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Physiology
语种	英语
出版者	GENERAL PHYSIOL AND BIOPHYSICS
WOS记录号	WOS:000240213000004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378528
专题	中国科学院大学
通讯作者	Zhang, G. -J.
作者单位	1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Hunan Agr Univ, Changsha, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China 4.Univ Sci & Technol Beijing, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Yi, Y. P.,Wang, X.,Zhang, G.,et al. Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+[J]. General physiology and biophysics,2006,25(2):149-160.
APA	Yi, Y. P.,Wang, X.,Zhang, G.,Fu, T. -S.,&Zhang, G. -J..(2006).Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+.General physiology and biophysics,25(2),149-160.
MLA	Yi, Y. P.,et al."Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to k+ and h+".General physiology and biophysics 25.2(2006):149-160.

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