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	Contributions of cysteine residues in zn2 to zinc fingers and thiol-disulfide oxidoreductase activities of chaperone dnaj
	Shi, YY; Tang, W; Hao, SF; Wang, CC
刊名	Biochemistry
	2005-02-08
卷号	44期号:5页码:1683-1689
ISSN号	0006-2960
DOI	10.1021/bi0480943
通讯作者	Wang, cc()
英文摘要	Escherichia coli dnaj, possessing both chaperone and thiol-disulfide oxidoreductase activities, is a homodimeric hsp40 protein. each subunit contains four copies of a sequence of -cxxcxgxg-, which coordinate with two zn(ii) ions to form an unusual topology of two c-4-type zinc fingers, (cdvczn)-d-144-zn-147(ii)(cnkc200)-n-197 (zn1) and (cptczn)-p-161-zn-164(ii)(cphc116)-p-183(zn2). studies on five dnaj mutants with cys in zn2 replaced by his or ser (c183h, c186h, c161h/c183h, c164h/183h, and c161s/ c164s) reveal that substitutions of one or two cys residues by his or ser have little effect on the general conformation and association property of the molecule. replacement of two cys residues by his does not interfere with the zinc coordination. however, replacement of two cys by ser results in a significant decrease in the proportion of coordinated zn(ii), although the unique zinc finger topology is retained. the mutants of c183h, c186h, and c161s/c164s display full disulfide reductase activity of wild-type dnaj, while c 161 h/c183h and c164h/183h exhibit severe defect in the activity. all of the mutations do not substantially affect the chaperone activity. the results indicate that the motif of -cxxc- is critical to form an active site and indispensable to the thiol-disulfide oxidoreductase activity of dnaj. each -cxxc- motif in zn2 but not in zn1 functions as an active site.
WOS关键词	ESCHERICHIA-COLI ; NUCLEOCAPSID PROTEIN ; BINDING ; THIOREDOXIN ; ISOMERASE ; PURIFICATION ; DISSOCIATION ; REDUCTION ; DOMAIN ; SITES
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000226802000031
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378338
专题	中国科学院大学
通讯作者	Wang, CC
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Shi, YY,Tang, W,Hao, SF,et al. Contributions of cysteine residues in zn2 to zinc fingers and thiol-disulfide oxidoreductase activities of chaperone dnaj[J]. Biochemistry,2005,44(5):1683-1689.
APA	Shi, YY,Tang, W,Hao, SF,&Wang, CC.(2005).Contributions of cysteine residues in zn2 to zinc fingers and thiol-disulfide oxidoreductase activities of chaperone dnaj.Biochemistry,44(5),1683-1689.
MLA	Shi, YY,et al."Contributions of cysteine residues in zn2 to zinc fingers and thiol-disulfide oxidoreductase activities of chaperone dnaj".Biochemistry 44.5(2005):1683-1689.

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