| Loss of function of osdcl1 affects microrna accumulation and causes developmental defects in rice | |
| Liu, B; Li, PC; Li, X; Liu, CY; Cao, SY; Chu, CC; Cao, XF | |
| 刊名 | Plant physiology
 |
| 2005-09-01 | |
| 卷号 | 139期号:1页码:296-305 |
| ISSN号 | 0032-0889 |
| 通讯作者 | Cao, xf(xfcao@genetics.ac.cn) |
| 英文摘要 | Micrornas (mirnas) and small interfering rnas (sirnas) are two types of noncoding rnas involved in developmental regulation, genome maintenance, and defense in eukaryotes. the activity of dicer or dicer-like (dcl) proteins is required for the maturation of mirnas and sirnas. in this study, we cloned and sequenced 66 candidate rice (oryza sativa) mirnas out of 1,650 small rna sequences (19 to approximately 25 nt), and they could be further grouped into 21 families, 12 of which are newly identified and three of which, osmir528, osmir529, and osmir530, have been confirmed by northern blot. to study the function of rice dcl proteins (osdcls) in the biogenesis of mirnas and sirnas, we searched genome databases and identified four osdcls. an rna interference approach was applied to knock down two osdcls, osdcl1 and osdcl4, respectively. strong loss of function of osdcl1ir transformants that expressed inverted repeats of osdcl1 resulted in developmental arrest at the seedling stage, and weak loss of function of osdcl1ir transformants caused pleiotropic developmental defects. moreover, all mirnas tested were greatly reduced in osdcl1ir but not osdcl4ir transformants, indicating that osdcl1 plays a critical role in mirna processing in rice. in contrast, the production of sirna from transgenic inverted repeats and endogenous cento regions were not affected in either osdcl1ir or osdcl4ir transformants, suggesting that the production of mirnas and sirnas is via distinct osdcls. |
| WOS关键词 | DOUBLE-STRANDED-RNA ; BINDING-PROTEIN HYL1 ; ARABIDOPSIS-THALIANA ; PLANT DEVELOPMENT ; DNA METHYLATION ; INTERFERING RNA ; LEAF POLARITY ; SIRNA ; GENES ; MIRNA |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| WOS记录号 | WOS:000231765300028 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2378050 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cao, XF |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Plant Gene Res, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.China Agr Univ, Beijing 100094, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, B,Li, PC,Li, X,et al. Loss of function of osdcl1 affects microrna accumulation and causes developmental defects in rice[J]. Plant physiology,2005,139(1):296-305. |
| APA | Liu, B.,Li, PC.,Li, X.,Liu, CY.,Cao, SY.,...&Cao, XF.(2005).Loss of function of osdcl1 affects microrna accumulation and causes developmental defects in rice.Plant physiology,139(1),296-305. |
| MLA | Liu, B,et al."Loss of function of osdcl1 affects microrna accumulation and causes developmental defects in rice".Plant physiology 139.1(2005):296-305. |
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