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	The composition and distribution of metal clusters in the mofe protein from a nifz deletion strain (dj 194) of azotobacter vinelandii
	Zhou, HN; Zhang, CX; Zhao, Y; Bian, SM; Ren, F; Wang, HP; Huang, JF
刊名	Chinese science bulletin
	2005-07-01
卷号	50期号:13页码:1342-1347
关键词	Azotobacter vinelandii Nifz deletion Iron-molybdenum protein Metal cluster Electronic paramagnetic resonance (epr) Circular dichrosim (cd)
ISSN号	1001-6538
DOI	10.1360/982005-417
通讯作者	Huang, jf()
英文摘要	Through the anaerobic chromatography on the columns of deae 52, q-sepharose and sephacryl s-200, a nitrogenase mofe protein (delta nifz av1) was obtained from a nijz deleted mutant of azotobacter vinelandii (stain dj194). the results of western blotting after anoxic native electrophoresis and sds-page showed that delta nijz av1 was similar to wild type mofe protein (op av1) at the electrophoretic mobility, molecular weight and subunit composition. furthermore, delta nijz av1 was also similar to op av1 at the molybdenum content, epr signal (g approximate to 4.3, 3.65 and 2.01), and the molar extinction coefficient (delta epsilon) of circular dichroism (cd) at 660 nm region. all of these indicated that, besides having the same alpha(2)beta(2) composition as op av1, the delta nifz av1 also contained equal amount of reductive femoco in the spin state of s=3/2 to op av1. however, the iron content and substrate (c2h2, h+ and n-2)-reduction activity of delta nijz av1 were 74% and 46% -50% of those of op av1, respectively. furthermore, the delta epsilon at around 450 nm, which reflects p-cluster in av1, was obviously lower than that of op av1. it suggested that the difference between delta nifz av1 and op av1 resulted from p-cluster rather than femoco, and from the half number of p-cluster in delta nijz av1, but the composition or redox state of p-cluster in delta nijz av1 were not changed. thus it could propose that delta nijz av1 is composed of two different up subunit pairs. one is a femoco- and p-cluster-containing pair, and the other is a p-cluster-deficient but femoco-containing pair. since the deletion of nijz gene leads to the deficiency of only one of two p-clusters in a alpha(2)beta(2) tetramer, the assembly of p-cluster may not simply depend on one gene product, and so a possible mechanism of nifz is supposed here.
WOS关键词	MAGNETIC CIRCULAR-DICHROISM ; IRON-MOLYBDENUM COFACTOR ; GENETIC-ANALYSIS ; KLEBSIELLA-PNEUMONIAE ; NITROGENASE ; PURIFICATION ; CHROOCOCCUM
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000231858000009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377788
专题	中国科学院大学
通讯作者	Huang, JF
作者单位	1.Chinese Acad Sci, Key Lab Photosynth & Environm Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing 100080, Peoples R China 4.China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China 5.Fujian Normal Univ, Bioengn Coll, Fuzhou 350007, Peoples R China
推荐引用方式 GB/T 7714	Zhou, HN,Zhang, CX,Zhao, Y,et al. The composition and distribution of metal clusters in the mofe protein from a nifz deletion strain (dj 194) of azotobacter vinelandii[J]. Chinese science bulletin,2005,50(13):1342-1347.
APA	Zhou, HN.,Zhang, CX.,Zhao, Y.,Bian, SM.,Ren, F.,...&Huang, JF.(2005).The composition and distribution of metal clusters in the mofe protein from a nifz deletion strain (dj 194) of azotobacter vinelandii.Chinese science bulletin,50(13),1342-1347.
MLA	Zhou, HN,et al."The composition and distribution of metal clusters in the mofe protein from a nifz deletion strain (dj 194) of azotobacter vinelandii".Chinese science bulletin 50.13(2005):1342-1347.

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