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	Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration
	Li, YD; Shi, XL; Parry, G; Chen, LQ; Callahan, JA; Mazar, AP; Huang, MD
刊名	Protein and peptide letters
	2005-10-01
卷号	12期号:7页码:655-658
关键词	Hydrogen peroxide Crystal optimization Upar Antibody
ISSN号	0929-8665
通讯作者	Huang, md()
英文摘要	Optimization of protein crystal formation is often a necessary step leading to diffraction-quality crystals to enable collection of a full x-ray data set. typical protein crystal optimization involves screening different components, e.g., ph, precipitants, and additives of the precipitant solution. here we present an example using an inhibitory antibody of urokinase plasminogen activator receptor (upar) where such procedures did not yield diffracting crystals. in contrast, it was the treatment of the protein with hydrogen peroxide incubation and the protein concentration reduction that were found to be key factors in obtaining diffracting crystals. final crystals diffracted to 1.75 angstrom, and belong to orthorhombic p2(1)2(1)2(1) space group with unit cell parameters a=37.162 angstrom, b=84.474 angstrom, c=134.030 angstrom, and contain one molecule of fab fragment of anti-uro kinase receptor antibody in the asymmetric unit.
WOS关键词	INTEGRIN FUNCTION ; CRYSTALLIZATION ; BINDING ; CANCER ; IDENTIFICATION ; VITRONECTIN ; MIGRATION ; THERAPY ; TARGET ; SITE
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000231958900008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377661
专题	中国科学院大学
通讯作者	Huang, MD
作者单位	1.Chinese Acad Sci, Fujian Inst Res Struct Matter, Grad Sch, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China 2.Attenuon LLC, San Diego, CA 92121 USA 3.Univ Alabama, Struct Biol Lab, Dept Chem, Grad Program Biotechnol, Huntsville, AL 35899 USA 4.Univ Alabama, Struct Biol Lab, Dept Chem, Grad Program Chem, Huntsville, AL 35899 USA 5.Univ Alabama, Struct Biol Lab, Dept Chem, Grad Program Mat Sci, Huntsville, AL 35899 USA
推荐引用方式 GB/T 7714	Li, YD,Shi, XL,Parry, G,et al. Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration[J]. Protein and peptide letters,2005,12(7):655-658.
APA	Li, YD.,Shi, XL.,Parry, G.,Chen, LQ.,Callahan, JA.,...&Huang, MD.(2005).Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration.Protein and peptide letters,12(7),655-658.
MLA	Li, YD,et al."Optimization of crystals of an inhibitory antibody of urokinase plasminogen activator receptor (upar) with hydrogen peroxide and low protein concentration".Protein and peptide letters 12.7(2005):655-658.

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