| Voltammetric determination of surface-confined biomolecules with n-(2-ethyl-ferrocene)maleimide | |
| Wang, YF; Zhu, X; Hu, ZB; Wang, JX; Zhou, FM | |
| 刊名 | Electroanalysis
 |
| 2005-12-01 | |
| 卷号 | 17期号:23页码:2163-2169 |
| 关键词 | Redox labels Voltammetry Quartz crystal microbalance N-(2-ethyl-ferrocene)maleimide Dna hybridization |
| ISSN号 | 1040-0397 |
| DOI | 10.1002/elan.200503347 |
| 通讯作者 | Wang, jx() |
| 英文摘要 | A thiol-specific electroactive cross-linker, n-(2-ethyl-ferrocene) maleimide (fc-mi), has been used to tag surface-confined peptides containing cysteine residues or oligodeoxynucleotides (odns) whose 3' ends have been modified with thiol groups. the peptides studied herein include both the oxidized and reduced forms of glutathione and a hexapeptide. cyclic voltammograms (cvs) of the fc-mi groups attached to the surfaces were used to quantify the total number of cysteine residues that are tagged and/or call undergo facile electron transfer reactions with the underlying electrodes. a quartz crystal microbalance was used in conjunction with cv to estimate the total number of cysteine groups labeled by fc-mi per peptide molecule. by comparing to mass spectrometric studies, it is confirmed that not all of the fc-mi linked to the cysteine groups can participate in file electron transfer reactions. the methodology is further extended to the determination of odn samples in a sandwich assay wherein the thiol linker oil the 3' end call be tagged with fc-mi. the analytical performance was evaluated through determinations of a complementary odn target and targets with varying numbers of mismatching bases. odn samples as low as 10 fmol can be detected. such a low detection level is remarkable considering that no signal amplification scheme is involved in the current method. the approach is shown to be sequence- and/or structure-specific and does not require sophisticated instrumentation and complex experimental procedure. |
| WOS关键词 | QUARTZ-CRYSTAL MICROBALANCE ; SELF-ASSEMBLED MONOLAYERS ; DNA-BINDING ; REDOX MODULATION ; P53 ; HYBRIDIZATION ; PROTEINS ; IDENTIFICATION ; ATTACHMENT ; OXIDATION |
| WOS研究方向 | Chemistry ; Electrochemistry |
| WOS类目 | Chemistry, Analytical ; Electrochemistry |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000234031500008 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2377213 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, JX |
| 作者单位 | 1.Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Peoples R China 2.Chinese Acad Sci, Grad Sch, Dept Chem, Beijing 100039, Peoples R China 3.Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA |
| 推荐引用方式 GB/T 7714 | Wang, YF,Zhu, X,Hu, ZB,et al. Voltammetric determination of surface-confined biomolecules with n-(2-ethyl-ferrocene)maleimide[J]. Electroanalysis,2005,17(23):2163-2169. |
| APA | Wang, YF,Zhu, X,Hu, ZB,Wang, JX,&Zhou, FM.(2005).Voltammetric determination of surface-confined biomolecules with n-(2-ethyl-ferrocene)maleimide.Electroanalysis,17(23),2163-2169. |
| MLA | Wang, YF,et al."Voltammetric determination of surface-confined biomolecules with n-(2-ethyl-ferrocene)maleimide".Electroanalysis 17.23(2005):2163-2169. |
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