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	Rational design of highly potent hiv-1 fusion inhibitory proteins: implication for developing antiviral therapeutics
	Ni, L; Gao, GF; Tien, P
刊名	Biochemical and biophysical research communications
	2005-07-08
卷号	332期号:3页码:831-836
关键词	Hiv-1 fusion inhibitors Gp41 Protein inhibitors Heptad-repeat regions
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2005.05.037
通讯作者	Gao, gf()
英文摘要	Recombinant protein containing one heptad-repeat 1 (hr1) segment and one hr2 segment of the hiv-1 gp41 (hr1-hr2) has been shown to fold into thermally stable six-helix bundle, representing the fusogenic core of gp41. in this study, we have used the fusogenic core as a scaffold to design hiv-1 fusion inhibitory proteins by linking another hr1 to the c terminus of hr1-hr2 (hr121) or additional hr2 to the n terminus of hr1-hr2 (hr212). both recombinant proteins could be abundantly and solubly expressed and easily purified, exhibiting high stability and potent inhibitory activity on hiv-1 fusion with ic50 values of 16.2 +/- 2.8 and 2.8 +/- 0.63 nm, respectively. these suggest that these rationally designed proteins can be further developed as novel anti-hiv-1 therapeutics. (c) 2005 elsevier inc. all rights reserved.
WOS关键词	VIRAL MEMBRANE-FUSION ; VIRUS TYPE-1 GP41 ; TRANSMEMBRANE GLYCOPROTEIN ; ENTRY ; INFECTION ; DOMAIN ; PEPTIDE ; ENFUVIRTIDE ; SUBDOMAIN ; CORE
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000229664500028
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377191
专题	中国科学院大学
通讯作者	Gao, GF
作者单位	1.Chinese Acad Sci, Inst Microbiol, Dept Mol Virol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Ni, L,Gao, GF,Tien, P. Rational design of highly potent hiv-1 fusion inhibitory proteins: implication for developing antiviral therapeutics[J]. Biochemical and biophysical research communications,2005,332(3):831-836.
APA	Ni, L,Gao, GF,&Tien, P.(2005).Rational design of highly potent hiv-1 fusion inhibitory proteins: implication for developing antiviral therapeutics.Biochemical and biophysical research communications,332(3),831-836.
MLA	Ni, L,et al."Rational design of highly potent hiv-1 fusion inhibitory proteins: implication for developing antiviral therapeutics".Biochemical and biophysical research communications 332.3(2005):831-836.

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