QQ客服 官方微博 反馈留言

	Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy
	Jiang, YX; Wang, J; Fang, XH; Bai, CL
刊名	Journal of nanoscience and nanotechnology
	2004-07-01
卷号	4期号:6页码:611-615
关键词	Afm Rupture force Aptamer Immunoglobulin e (ige)
ISSN号	1533-4880
通讯作者	Fang, xh()
英文摘要	We have studied the effect of metal ions on the specific interaction between a protein, immunoglobulin e (ige), and its 37-nt dna aptamer with atomic force microscopy (afm). protein aptamers are a new class of synthetic single-stranded dna/rna oligonucleotide generated from in vitro selection to selectively bind with target proteins. the ige aptamers have been developed and are expected to be promising reagents in ige detection and new anti-allergic drug development. it is known that the presence of metal ions in the buffer usually has a strong effect on the affinity of single-stranded dna for protein. in this work, the effect of two representative monovalent ion and divalent ion on the binding of ige and the aptamer has been studied at the single-molecule level. the results from the afm force measurements show that the metal ions not only reduce the single-molecular rupture force but also reduce the number of bonds formed between ige and the aptamer.
WOS关键词	MOLECULAR APTAMER ; ANTIBODY ; LIGANDS ; RECOGNITION ; BEACONS ; DNA ; ORGANOSILANES ; POISSON ; EVENTS ; BIOTIN
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
语种	英语
出版者	AMER SCIENTIFIC PUBLISHERS
WOS记录号	WOS:000224698300011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376957
专题	中国科学院大学
通讯作者	Fang, XH
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Jiang, YX,Wang, J,Fang, XH,et al. Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy[J]. Journal of nanoscience and nanotechnology,2004,4(6):611-615.
APA	Jiang, YX,Wang, J,Fang, XH,&Bai, CL.(2004).Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy.Journal of nanoscience and nanotechnology,4(6),611-615.
MLA	Jiang, YX,et al."Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy".Journal of nanoscience and nanotechnology 4.6(2004):611-615.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。