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	Functional architecture of two exclusively late stage pollen-specific promoters in rice (oryza sativa l.)
	Yan, Shuo1,2; Wang, Zhongni1,2; Liu, Yuan1; Li, Wei3; Wu, Feng1; Lin, Xuelei1; Meng, Zheng1
刊名	Plant molecular biology
	2015-07-01
卷号	88期号:4-5页码:415-428
关键词	Pollen-specific promoter Rice Cis-element Functional architecture Stable transformation
ISSN号	0167-4412
DOI	10.1007/s11103-015-0331-0
通讯作者	Meng, zheng(zhmeng@ibcas.ac.cn)
英文摘要	Late stage pollen-specific promoters are important tools in crop molecular breeding. several such promoters, and their functional motifs, have been well characterized in dicotyledonous plants such as tomato and tobacco. however, knowledge about the functional architecture of such promoters is limited in the monocotyledonous plant rice. here, pollen-late-stage-promoter 1 (plp1) and pollen-late-stage-promoter 2 (plp2) were characterized using a stable transformation system in rice. histochemical staining showed that the two promoters exclusively drive gus expression in late-stage pollen grains in rice. 5' deletion analysis revealed that four regions, including the -1159 to -720 and the -352 to -156 regions of plp1 and the -740 to -557 and the -557 to -339 regions of plp2, are important in maintaining the activity and specificity of these promoters. motif mutation analysis indicated that 'agaaa' and 'caat' motifs in the -740 to -557 region of plp2 act as enhancers in the promoter. gain of function experiments indicated that the novel ta-rich motif 'tacataa' and 'tattcat' in the core region of the plp1 and plp2 promoters is necessary, but not sufficient, for pollen-specific expression in rice. our results provide evidence that the enhancer motif 'agaaa' is conserved in the pollen-specific promoters of both monocots and eudicots, but that some functional architecture characteristics are different.
WOS关键词	TISSUE-SPECIFIC EXPRESSION ; CIS-REGULATORY ELEMENTS ; MALE-STERILITY ; TRANSGENIC RICE ; GENE-EXPRESSION ; MESSENGER-RNAS ; PLANTS ; ARABIDOPSIS ; ANTHER ; TOBACCO
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000357052100008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376380
专题	中国科学院大学
通讯作者	Meng, Zheng
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 3.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China
推荐引用方式 GB/T 7714	Yan, Shuo,Wang, Zhongni,Liu, Yuan,et al. Functional architecture of two exclusively late stage pollen-specific promoters in rice (oryza sativa l.)[J]. Plant molecular biology,2015,88(4-5):415-428.
APA	Yan, Shuo.,Wang, Zhongni.,Liu, Yuan.,Li, Wei.,Wu, Feng.,...&Meng, Zheng.(2015).Functional architecture of two exclusively late stage pollen-specific promoters in rice (oryza sativa l.).Plant molecular biology,88(4-5),415-428.
MLA	Yan, Shuo,et al."Functional architecture of two exclusively late stage pollen-specific promoters in rice (oryza sativa l.)".Plant molecular biology 88.4-5(2015):415-428.

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