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	Hydrophobic tagging-assisted n-termini enrichment for in-depth n-terminome analysis
	Chen, Lingfan1,2; Shan, Yichu1; Weng, Yejing1,2; Sui, Zhigang1; Zhang, Xiaodan1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
刊名	Analytical chemistry
	2016-09-06
卷号	88期号:17页码:8390-8395
ISSN号	0003-2700
DOI	10.1021/acs.analchem.6b02453
通讯作者	Zhang, lihua(lihuazhang@dicp.ac.cn)
英文摘要	The analysis of protein n-termini is of great importance for understanding the protein function and elucidating the proteolytic processing. herein, we develop a negative enrichment strategy, termed as hydrophobic tagging-assisted n-termini enrichment (hytane) to achieve a global n-terminome analysis. the hytane strategy showed a high efficiency in hydrophobic tagging and c18 material-assisted depletion using bovine serum albumin (bsa) as the sample. this strategy was applied to n-termini profiling from s. cerevisiae cell lysates and enabled the identification of 1096 protein n-termini, representing the largest n-terminome data set of s. cerevisiae. the identified n-terminal peptides accounted for 99% of all identified peptides, and no deficiency in acidic, histidine (his)-containing, and his-free n-terminal peptides was observed. the presented hytane strategy is therefore a highly selective, efficient, and unbiased strategy for the large scale n-terminome analysis. furthermore, using the hytane strategy, we identified 329 cleavage sites and 291 substrates of caspases in jurkat cells, demonstrating the great promise of hytane strategy for protease research. data are available via proteomexchange with identifier pxd004690.
WOS关键词	POSITIONAL PROTEOMICS ; SELECTIVE ISOLATION ; MASS-SPECTROMETRY ; CLEAVAGE SITES ; PEPTIDES ; PROTEINS ; ACETYLATION ; COMPLEX ; APOPTOSIS ; STRATEGY
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000382805900010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376297
专题	中国科学院大学
通讯作者	Zhang, Lihua
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Lingfan,Shan, Yichu,Weng, Yejing,et al. Hydrophobic tagging-assisted n-termini enrichment for in-depth n-terminome analysis[J]. Analytical chemistry,2016,88(17):8390-8395.
APA	Chen, Lingfan.,Shan, Yichu.,Weng, Yejing.,Sui, Zhigang.,Zhang, Xiaodan.,...&Zhang, Yukui.(2016).Hydrophobic tagging-assisted n-termini enrichment for in-depth n-terminome analysis.Analytical chemistry,88(17),8390-8395.
MLA	Chen, Lingfan,et al."Hydrophobic tagging-assisted n-termini enrichment for in-depth n-terminome analysis".Analytical chemistry 88.17(2016):8390-8395.

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