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	Spatiotemporal relationships among d-serine, serine racemase, and d-amino acid oxidase during mouse postnatal development
	Wang, LZ; Zhiu, XZ
刊名	Acta pharmacologica sinica
	2003-10-01
卷号	24期号:10页码:965-974
关键词	D-amino acid oxidase Serine Serine racemase Inbred c57bl mice
ISSN号	1671-4083
通讯作者	Zhiu, xz(xzzhu@mail.shcnc.ac.cn)
英文摘要	Aim: to elucidate the spatiotemporal relationships among d-serine, serine racemase, and d-amino acid oxidase (ec 1.4.3.3; dao) in mouse cortex, striatum, cerebellum, heart, lung, liver, spleen, kidney, and skeletal muscle during mouse postnatal development. methods: the transcription levels of serine racemase and dao were assayed by reverse transcription-polymerase chain reaction (rt-pcr). the protein levels of serine racemase were examined by western blot. dao activities were assayed by colorimetric method. d-serine was measured by hplc. results: in cortex, striatum, and cerebellum, free d-serine increased drastically after birth and coincided well with the increase of serine racemase expression. however, among the 9 tissues examined, dao activities were detected only in cerebellum and kidney. during the 3rd week, dao activity in cerebellum and kidney increased dramatically, which concurred with the drastic decline of d-serine content in these tissues. on the other hand, while d-serine and serine racemase fall to trace level in cerebellum and kidney at the 3rd weekend, dao activities in these tissues increased continuously. conclusion: the free d-serine is mainly synthesized by serine racemase. however, novel mechanisms might be involved in d-serine deposition in mouse tissues with high level of d-serine and no detectable dao activity such as cortex and striatum. dao in cerebellum and kidney might have other physiological functions in addition to degrading d-amino acid.
WOS关键词	D-ASPARTATE RECEPTOR ; RAT-BRAIN ; NMDA RECEPTORS ; EXTRACELLULAR CONCENTRATION ; SYNAPSE ELIMINATION ; GLYCINE SITE ; D-ALANINE ; NEUROMODULATOR ; CEREBELLUM ; RODENTS
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
WOS类目	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
语种	英语
出版者	SHANGHAI INST MATERIA MEDICA
WOS记录号	WOS:000185749400003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376141
专题	武汉病毒研究所
通讯作者	Zhiu, XZ
作者单位	Chinese Acad Sci, Inst Biol Sci, Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Wang, LZ,Zhiu, XZ. Spatiotemporal relationships among d-serine, serine racemase, and d-amino acid oxidase during mouse postnatal development[J]. Acta pharmacologica sinica,2003,24(10):965-974.
APA	Wang, LZ,&Zhiu, XZ.(2003).Spatiotemporal relationships among d-serine, serine racemase, and d-amino acid oxidase during mouse postnatal development.Acta pharmacologica sinica,24(10),965-974.
MLA	Wang, LZ,et al."Spatiotemporal relationships among d-serine, serine racemase, and d-amino acid oxidase during mouse postnatal development".Acta pharmacologica sinica 24.10(2003):965-974.

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