| Ythdf2 destabilizes m(6)a-containing rna through direct recruitment of the ccr4-not deadenylase complex | |
| Du, Hao1,2,3; Zhao, Ya1,2,3; He, Jinqiu4; Zhang, Yao5; Xi, Hairui1,2,3,6; Liu, Mofang3; Ma, Jinbiao4; Wu, Ligang1,2,3 | |
| 刊名 | Nature communications
 |
| 2016-08-01 | |
| 卷号 | 7页码:11 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/ncomms12626 |
| 通讯作者 | Ma, jinbiao(majb@fudan.edu.cn) ; Wu, ligang(lgwu@sibcb.ac.cn) |
| 英文摘要 | Methylation at the n6 position of adenosine (m(6)a) is the most abundant rna modification within protein-coding and long noncoding rnas in eukaryotes and is a reversible process with important biological functions. yt521-b homology domain family (ythdf) proteins are the readers of m(6)a, the binding of which results in the alteration of the translation efficiency and stability of m(6)a-containing rnas. however, the mechanism by which ythdf proteins cause the degradation of m(6)a-containing rnas is poorly understood. here we report that m(6)a-containing rnas exhibit accelerated deadenylation that is mediated by the ccr4-not deadenylase complex. we further show that ythdf2 recruits the ccr4-not complex through a direct interaction between the ythdf2 n-terminal region and the sh domain of the cnot1 subunit, and that this recruitment is essential for the deadenylation of m(6)a-containing rnas by caf1 and ccr4. therefore, we have uncovered the mechanism of ythdf2-mediated degradation of m(6)a-containing rnas in mammalian cells. |
| WOS关键词 | EMBRYONIC STEM-CELLS ; MESSENGER-RNA ; NUCLEAR-RNA ; METHYLATION ; N-6-METHYLADENOSINE ; BINDING ; PROTEIN ; DOMAIN ; REVEALS ; DECAY |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000383664000001 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2376082 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma, Jinbiao; Wu, Ligang |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biol Sci,Inst Biochem & Cell Biol, State Key Lab Mol Biol,Natl Ctr Prot Sci Shanghai, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, CAS Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China 4.Fudan Univ, Sch Life Sci, Inst Plant Biol,Dept Biochem, State Key Lab Genet Engn,Collaborat Innovat Ctr G, Shanghai 200433, Peoples R China 5.Shanghai Inst Planned Parenthood Res, Shanghai 200032, Peoples R China 6.Shanghai Univ, Sch Life Sci, 333 Nanchen Rd, Shanghai 200444, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Hao,Zhao, Ya,He, Jinqiu,et al. Ythdf2 destabilizes m(6)a-containing rna through direct recruitment of the ccr4-not deadenylase complex[J]. Nature communications,2016,7:11. |
| APA | Du, Hao.,Zhao, Ya.,He, Jinqiu.,Zhang, Yao.,Xi, Hairui.,...&Wu, Ligang.(2016).Ythdf2 destabilizes m(6)a-containing rna through direct recruitment of the ccr4-not deadenylase complex.Nature communications,7,11. |
| MLA | Du, Hao,et al."Ythdf2 destabilizes m(6)a-containing rna through direct recruitment of the ccr4-not deadenylase complex".Nature communications 7(2016):11. |
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