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	Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna
	Zhang, Yi1,2; Liang, Zhen1,2; Zong, Yuan1,2; Wang, Yanpeng1,2; Liu, Jinxing1; Chen, Kunling1; Qiu, Jin-Long3; Gao, Caixia1
刊名	Nature communications
	2016-08-01
卷号	7页码:8
ISSN号	2041-1723
DOI	10.1038/ncomms12617
通讯作者	Gao, caixia(cxgao@genetics.ac.cn)
英文摘要	Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing crispr/cas9 introduced as dna or rna. this transient expression-based genome-editing system is highly efficient and specific for producing transgene-free and homozygous wheat mutants in the t0 generation. we demonstrate our protocol to edit genes in hexaploid bread wheat and tetraploid durum wheat, and show that we are able to generate mutants with no detectable transgenes. our methods may be applicable to other plant species, thus offering the potential to accelerate basic and applied plant genome-engineering research.
WOS关键词	NATURAL VARIATION ; CAS9 NUCLEASE ; COMMON WHEAT ; CROP PLANTS ; HUMAN-CELLS ; RICE ; SYSTEM ; GENE ; RIBONUCLEOPROTEINS ; SPECIFICITY
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000383663700001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375990
专题	中国科学院大学
通讯作者	Gao, Caixia
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yi,Liang, Zhen,Zong, Yuan,et al. Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna[J]. Nature communications,2016,7:8.
APA	Zhang, Yi.,Liang, Zhen.,Zong, Yuan.,Wang, Yanpeng.,Liu, Jinxing.,...&Gao, Caixia.(2016).Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna.Nature communications,7,8.
MLA	Zhang, Yi,et al."Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna".Nature communications 7(2016):8.

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