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	Purification and characterization of the acetyl-coa synthetase from mycobacterium tuberculosis
	Li, Ru2,3; Gu, Jing2; Chen, Peng4; Zhang, Zhiping2; Deng, Jiaoyu1; Zhang, XianEn2
刊名	Acta biochimica et biophysica sinica
	2011-11-01
卷号	43期号:11页码:891-899
关键词	Acetyl-coa synthetase Acetate Auto-acetylation Deacetylase Mycobacterium tuberculosis
ISSN号	1672-9145
DOI	10.1093/abbs/gmr076
通讯作者	Deng, jiaoyu(dengjy@wh.iov.cn)
英文摘要	Acetyl-coa (accoa) synthetase (acs) catalyzes the conversion of acetate into accoa, which is involved in many catabolic and anabolic pathways. although this enzyme has been studied for many years in many organisms, the properties of mycobacterium tuberculosis acs and the regulation of its activity remain unknown. here, the putative acs gene of m. tuberculosis h37rv (mt-acs) was expressed as a fusion protein with 6xhis-tag on the c-terminus in escherichia coli. the recombinant mt-acs protein was successfully purified and then its enzymatic characteristics were analyzed. the optimal ph and temperature, and the kinetic parameters of mt-acs were determined. to investigate whether mt-acs is regulated by lysine acetylation as reported for salmonella enterica acs, its mutant k617r was also generated. determination of the enzymatic activity suggests that lys-617 is critical for its function. we further demonstrated that mt-acs underwent auto-acetylation with acetate but not with accoa as the acetyl donor, which resulted in the decrease of its activity. coa, the substrate for accoa formation, inhibited the auto-acetylation. furthermore, the silent information regulator (sir2) of m. tuberculosis (mt-sir2) could catalyze mt-acs deacetylation, which resulted in activation of acs. these results may provide more insights into the physiological roles of mt-acs in m. tuberculosis central metabolism.
WOS关键词	COENZYME-A SYNTHETASE ; RESPONSE REGULATOR CHEY ; ESCHERICHIA-COLI ; SALMONELLA-ENTERICA ; AUTO-ACETYLATION ; ACYL ADENYLATES ; ENZYME ; ACETATE ; ACTIVATION ; LYSINE
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000296291000008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375967
专题	武汉病毒研究所
通讯作者	Deng, Jiaoyu
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Peoples R China 4.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China
推荐引用方式 GB/T 7714	Li, Ru,Gu, Jing,Chen, Peng,et al. Purification and characterization of the acetyl-coa synthetase from mycobacterium tuberculosis[J]. Acta biochimica et biophysica sinica,2011,43(11):891-899.
APA	Li, Ru,Gu, Jing,Chen, Peng,Zhang, Zhiping,Deng, Jiaoyu,&Zhang, XianEn.(2011).Purification and characterization of the acetyl-coa synthetase from mycobacterium tuberculosis.Acta biochimica et biophysica sinica,43(11),891-899.
MLA	Li, Ru,et al."Purification and characterization of the acetyl-coa synthetase from mycobacterium tuberculosis".Acta biochimica et biophysica sinica 43.11(2011):891-899.

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