QQ客服 官方微博 反馈留言

	Functional nicotinic acetylcholine receptors containing alpha 6 subunits are on gabaergic neuronal boutons adherent to ventral tegmental area dopamine neurons
	Yang, Kechun1; Buhlman, Lori2; Khan, Ghous M.3; Nichols, Robert A.3; Jin, Guozhang4; McIntosh, J. Michael5,6; Whiteaker, Paul2; Lukas, Ronald J.2; Wu, Jie1
刊名	Journal of neuroscience
	2011-02-16
卷号	31期号:7页码:2537-2548
ISSN号	0270-6474
DOI	10.1523/jneurosci.3003-10.2011
通讯作者	Wu, jie(jie.wu@chw.edu)
英文摘要	Diverse nicotinic acetylcholine receptor (nachr) subtypes containing different subunit combinations can be placed on nerve terminals or soma/dendrites in the ventral tegmental area (vta). nachr alpha 6 subunit message is abundant in the vta, but alpha 6(star)-nachr cellular localization, function, pharmacology, and roles in cholinergic modulation of dopaminergic (da) neurons within the vta are not well understood. here, we report evidence for alpha 6 beta 2(star)-nachr expression on gaba neuronal boutons terminating on vta da neurons. alpha-conotoxin (alpha-ctx) mii labeling coupled with immunocytochemical staining localizes putative alpha 6(star)-nachrs to presynaptic gabaergic boutons on acutely dissociated, rat vta da neurons. functionally, acetylcholine (ach) induces increases in the frequency of bicuculline-, picrotoxin-, and 4-aminopyridine-sensitive miniature ipscs (mipscs) mediated by gaba(a) receptors. these increases are abolished by alpha 6(star)-nachr-selective alpha-ctx mii or alpha-ctx pia (1 nm) but not by alpha 7 (10 nm methyllycaconitine) or alpha 4(star) (1 mu m dihydro-beta-erythroidine)-nachr-selective antagonists. ach also fails to increase mipsc frequency in vta da neurons prepared from nachr beta 2 knock-out mice. moreover, ach induces an alpha-ctx pia-sensitive elevation in intraterminal ca2+ in synaptosomes prepared from the rat vta. subchronic exposure to 500 nm nicotine reduces ach-induced gaba release onto the vta da neurons, as does 10 d of systemic nicotine exposure. collectively, these results indicate that alpha 6 beta 2(star)-nachrs are located on presynaptic gabaergic boutons within the vta and modulate gaba release onto da neurons. these presynaptic alpha 6 beta 2(star)-nachrs likely play important roles in nicotinic modulation of da neuronal activity.
WOS关键词	RAT-BRAIN ; SYNAPTIC MECHANISMS ; CONOTOXIN-MII ; NUCLEUS-ACCUMBENS ; SUBSTANTIA-NIGRA ; REWARD AREAS ; ADDICTION ; PHARMACOLOGY ; TRANSMISSION ; ANTAGONIST
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
语种	英语
出版者	SOC NEUROSCIENCE
WOS记录号	WOS:000287392400022
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375944
专题	武汉病毒研究所
通讯作者	Wu, Jie
作者单位	1.St Josephs Hosp, Barrow Neurol Inst, Div Neurol, Phoenix, AZ 85013 USA 2.St Josephs Hosp, Barrow Neurol Inst, Div Neurobiol, Phoenix, AZ 85013 USA 3.Univ Hawaii, Dept Physiol & Pharmacol, John A Burns Sch Med, Honolulu, HI 96813 USA 4.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 201203, Peoples R China 5.Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA 6.Univ Utah, Dept Psychiat, Salt Lake City, UT 84112 USA
推荐引用方式 GB/T 7714	Yang, Kechun,Buhlman, Lori,Khan, Ghous M.,et al. Functional nicotinic acetylcholine receptors containing alpha 6 subunits are on gabaergic neuronal boutons adherent to ventral tegmental area dopamine neurons[J]. Journal of neuroscience,2011,31(7):2537-2548.
APA	Yang, Kechun.,Buhlman, Lori.,Khan, Ghous M..,Nichols, Robert A..,Jin, Guozhang.,...&Wu, Jie.(2011).Functional nicotinic acetylcholine receptors containing alpha 6 subunits are on gabaergic neuronal boutons adherent to ventral tegmental area dopamine neurons.Journal of neuroscience,31(7),2537-2548.
MLA	Yang, Kechun,et al."Functional nicotinic acetylcholine receptors containing alpha 6 subunits are on gabaergic neuronal boutons adherent to ventral tegmental area dopamine neurons".Journal of neuroscience 31.7(2011):2537-2548.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。