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	Detection of zika virus by sybr green one-step real-time rt-pcr
	Xu, Ming-Yue1,2; Liu, Si Qing1; Deng, Cheng-Lin1; Zhang, Qiu-Yan1,2; Zhang, Bo1
刊名	Journal of virological methods
	2016-10-01
卷号	236页码:93-97
关键词	Zika virus Real-time rt-pcr Sybr green
ISSN号	0166-0934
DOI	10.1016/j.jviromet.2016.07.014
通讯作者	Zhang, bo(zhangbo@wh.iov.cn)
英文摘要	The ongoing zika virus (zikv) outbreak has rapidly spread to new areas of americas, which were the first transmissions outside its traditional endemic areas in africa and asia. due to the link with newborn defects and neurological disorder, numerous infected cases throughout the world and various mosquito vectors, the virus has been considered to be an international public health emergency. in the present study, we developed a sybr green based one-step real-time rt-pcr assay for rapid detection of zikv. our results revealed that the real-time assay is highly specific and sensitive in detection of zikv in cell samples. importantly, the replication of zikv at different time points in infected cells could be rapidly monitored by the real-time rt-pcr assay. specifically, the real-time rt-pcr showed acceptable performance in measurement of infectious zikv rna. this assay could detect zikv at a titer as low as 1 pfu/ml. the real-time rt-pcr assay could be a useful tool for further virology surveillance and diagnosis of zikv. (c) 2016 elsevier b.v. all rights reserved.
WOS关键词	JAPANESE ENCEPHALITIS-VIRUS ; AEDES-AEGYPTI ; FEVER VIRUS ; DENGUE ; TRANSMISSION ; NS2B ; RNA
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000383941900015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375907
专题	中国科学院大学
通讯作者	Zhang, Bo
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Ming-Yue,Liu, Si Qing,Deng, Cheng-Lin,et al. Detection of zika virus by sybr green one-step real-time rt-pcr[J]. Journal of virological methods,2016,236:93-97.
APA	Xu, Ming-Yue,Liu, Si Qing,Deng, Cheng-Lin,Zhang, Qiu-Yan,&Zhang, Bo.(2016).Detection of zika virus by sybr green one-step real-time rt-pcr.Journal of virological methods,236,93-97.
MLA	Xu, Ming-Yue,et al."Detection of zika virus by sybr green one-step real-time rt-pcr".Journal of virological methods 236(2016):93-97.

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