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	Matrix protein-specific iga antibody inhibits measles virus replication by intracellular neutralization
	Zhou, Dihan1,4; Zhang, Yan1,4; Li, Qiaoli1,4; Chen, Yaoqing1,2,3; He, Benxia1,4; Yang, Jingyi1,4; Tu, Haobo2,3; Lei, Lei2,3; Yan, Huimin1,2
刊名	Journal of virology
	2011-11-01
卷号	85期号:21页码:11090-11097
ISSN号	0022-538X
DOI	10.1128/jvi.00768-11
通讯作者	Yan, huimin(hmyan@wh.iov.cn)
英文摘要	Measles virus (mv) is still an imposing threat to public health. the matrix (m) protein has been shown not only to function as a structure block in the assembled mv virions, but also to regulate viral rna synthesis, playing an important role in mv's replication and assembly. in the present study, we generated a panel of igg monoclonal antibodies (mabs) against m protein and successfully obtained one iga mab (5h7) from the igg panel. employing the polarized vero cells grown in the two-chamber transwell model, we investigated whether m-specific 5h7 iga mab could suppress mv's replication and assembly. the data presented indicate that, while failing to show the activities of traditional neutralization and immune exclusion, m-specific iga mab was able to effectively inhibit viral replication by intracellular neutralization (78%), supporting the notion that the m protein is important for mv assembly and replication and implying that the m protein was an effective target antigen. the data also showed that mv had a long entry and assembly phase during viral replication, providing an extended window for iga intervention. the colocalization of m proteins and m-specific 5h7 iga mabs demonstrated that the intracellular neutralization was due to the direct binding of the m-specific 5h7 iga mabs to the m proteins. in summary, the present study has added another example showing that iga antibodies targeting internal viral antigens could proactively participate in mucosal immune protection by intracellular neutralization and has provided evidence that m protein might be included as a target antigen in future mv vaccine design.
WOS关键词	IMMUNOGLOBULIN-A ANTIBODIES ; EPITHELIAL-CELLS ; MONOCLONAL-ANTIBODIES ; HIV-1 REPLICATION ; MUCOSAL SURFACES ; UNITED-STATES ; FUSION ; TRANSCRIPTION ; PROTECTION ; ENVELOPE
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000296254400016
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375852
专题	武汉病毒研究所
通讯作者	Yan, Huimin
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Mucosal Immun Res Grp, Wuhan 430071, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Dihan,Zhang, Yan,Li, Qiaoli,et al. Matrix protein-specific iga antibody inhibits measles virus replication by intracellular neutralization[J]. Journal of virology,2011,85(21):11090-11097.
APA	Zhou, Dihan.,Zhang, Yan.,Li, Qiaoli.,Chen, Yaoqing.,He, Benxia.,...&Yan, Huimin.(2011).Matrix protein-specific iga antibody inhibits measles virus replication by intracellular neutralization.Journal of virology,85(21),11090-11097.
MLA	Zhou, Dihan,et al."Matrix protein-specific iga antibody inhibits measles virus replication by intracellular neutralization".Journal of virology 85.21(2011):11090-11097.

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