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	A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals
	Zhang, Wentao2,3; Wu, Di2,3; Wei, Jianghua2,3; Xiao, Gengfu1,2,3
刊名	Biotechnology letters
	2010-12-01
卷号	32期号:12页码:1933-1937
关键词	Hybridization Influenza virus Magnetic beads Quantum dots
ISSN号	0141-5492
DOI	10.1007/s10529-010-0379-5
通讯作者	Xiao, gengfu(xiaogf@wh.iov.cn)
英文摘要	A new method to detect h5 influenza virus using quantum dots (qds) and magnetic beads (mbs) is described. qds conjugated with oligonucleotide probes were used to produce fluorescent signals and mbs, that were also conjugated with probes, were used to isolate and concentrate the signals. target viral rnas led to a sandwich hybridization between the functionalized qds and mbs. one-step hybridization facilitated the subtype determination. as little as 0.1 ng viral rna could be detected.
WOS关键词	DNA ; PCR
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000285912900025
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375835
专题	武汉病毒研究所
通讯作者	Xiao, Gengfu
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wentao,Wu, Di,Wei, Jianghua,et al. A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals[J]. Biotechnology letters,2010,32(12):1933-1937.
APA	Zhang, Wentao,Wu, Di,Wei, Jianghua,&Xiao, Gengfu.(2010).A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals.Biotechnology letters,32(12),1933-1937.
MLA	Zhang, Wentao,et al."A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals".Biotechnology letters 32.12(2010):1933-1937.

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