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	The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas
	Liu, Zhaoqun1,3; Zhou, Zhi1; Wang, Lingling2; Dong, Wenjing1; Qiu, Limei1; Song, Linsheng2
刊名	Developmental and comparative immunology
	2016-12-01
卷号	65页码:139-148
关键词	Crassostrea gigas Acetylcholine Muscarinic acetylcholine receptor Tnf pathway Immune regulation
ISSN号	0145-305X
DOI	10.1016/j.dci.2016.07.003
通讯作者	Wang, lingling(wanglingling@ms.qdio.ac.cn) ; Song, linsheng(lshsong@dlou.edu.cn)
英文摘要	Muscarinic receptors, which selectively take muscarine as their ligand, are critical for the immunological and physiological processes in animals. in the present study, the open region frame (orf) of a homologue of muscarinic acetylcholine (ach) receptor (machr) was amplified from oyster crassostrea gigas (named as cgmachr-1), whose full length was 1983 bp and the protein it encoded contained 660 amino acids with a seven transmembrane region. phylogeny analysis suggested that cgmachr-1 shared homology with m-5 muscarinic receptor found in invertebrates including habropoda laboriosa, acromyrmex echinatior and echinococcus granulosus. after cell transfection of cgrnachr-1 into hek293t cells and ach incubation, the level of intracellular ca2+ and camp increased significantly (p < 0.05). such trend could be reverted with the addition of m-3 and m-5 muscarinic receptor antagonists damp and dar. the cgmachr-1 transcripts were ubiquitously detectable in seven different tissues with the maximal expression level in adductor muscle. when the oysters received lps stimulation, cgmachr-1 mrna expression in haemocyte was increased to the highest level (6.05-fold, p < 0.05) at 24 h, while blocking cgmachr-1 using receptor antagonists before lps stimulation promoted the expression of oyster tnf, resulting in the increase of haemocyte apoptosis index. these results suggested that cgmachr-1 was the key molecule in cholinergic neuroendocrine-immune system contributing to the regulation of tnf expression and apoptosis process. (c) 2016 elsevier ltd. all rights reserved.
WOS关键词	SCALLOP CHLAMYS-FARRERI ; VIBRIO-ANGUILLARUM CHALLENGE ; ANTIINFLAMMATORY PATHWAY ; NERVOUS-SYSTEM ; ROS GENERATION ; T-LYMPHOCYTE ; IMMUNOMODULATION ; LOCALIZATION ; INFLAMMATION ; MODULATION
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000384388400016
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375807
专题	中国科学院大学
通讯作者	Wang, Lingling; Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas[J]. Developmental and comparative immunology,2016,65:139-148.
APA	Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,Dong, Wenjing,Qiu, Limei,&Song, Linsheng.(2016).The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas.Developmental and comparative immunology,65,139-148.
MLA	Liu, Zhaoqun,et al."The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas".Developmental and comparative immunology 65(2016):139-148.

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