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	An auto-biotinylated bifunctional protein nanowire for ultra-sensitive molecular biosensing
	Men, Dong1,4; Zhang, Zhi-Ping1; Guo, Yong-Chao2; Zhu, Duan-Hao1,4; Bi, Li-Jun3; Deng, Jiao-Yu1; Cui, Zong-Qiang1; Wei, Hong-Ping1; Zhang, Xian-En1
刊名	Biosensors & bioelectronics
	2010-12-15
卷号	26期号:4页码:1137-1141
关键词	Auto-biotinylated Self-assembly Amyloid Bifunctional protein nanowire Ultra-sensitivity molecular biosensing Immunoassay
ISSN号	0956-5663
DOI	10.1016/j.bios.2010.07.103
通讯作者	Zhang, xian-en(x.zhang@wh.iov.cn)
英文摘要	In order to obtain an ultra-sensitive molecular biosensor, we designed an auto-biotinylated bifunctional protein nanowire (bfpnw) based on the self-assembly of a yeast amyloid protein, sup35, to which protein g and a biotin acceptor peptide (bap) were genetically fused. these auto-biotinylated bfpnws can transfer hundreds of commercially available diagnostic enzymes to an antigen-antibody complex via the biotin-avidin system, greatly enhancing the sensitivity of immune-biosensing. compared to our previously reported seeding-induced bfpnws (men et al., 2009), these auto-biotinylated bfpnws gave greater signal amplification, reduced non-specific binding and improved stability. the auto-biotinylated self-assembled bfpnw molecular biosensors were applied to detect yersinia pestis (y. pestis) f1 antigen and showed a 2000- to 4000-fold increase in sensitivity compared to traditional immunoassays, demonstrating the potential use of these self-assembling protein nanowires in biosensing. (c) 2010 elsevier b.v. all rights reserved.
WOS关键词	ESCHERICHIA-COLI ; BINDING ; SYSTEM
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000286403400003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375725
专题	武汉病毒研究所
通讯作者	Zhang, Xian-En
作者单位	1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Men, Dong,Zhang, Zhi-Ping,Guo, Yong-Chao,et al. An auto-biotinylated bifunctional protein nanowire for ultra-sensitive molecular biosensing[J]. Biosensors & bioelectronics,2010,26(4):1137-1141.
APA	Men, Dong.,Zhang, Zhi-Ping.,Guo, Yong-Chao.,Zhu, Duan-Hao.,Bi, Li-Jun.,...&Zhang, Xian-En.(2010).An auto-biotinylated bifunctional protein nanowire for ultra-sensitive molecular biosensing.Biosensors & bioelectronics,26(4),1137-1141.
MLA	Men, Dong,et al."An auto-biotinylated bifunctional protein nanowire for ultra-sensitive molecular biosensing".Biosensors & bioelectronics 26.4(2010):1137-1141.

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