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	Actin dynamics regulate multiple endosomal steps during kaposi's sarcoma-associated herpesvirus entry and trafficking in endothelial cells
	Greene, Whitney1,2; Gao, Shou-Jiang1,2,3,4,5
刊名	Plos pathogens
	2009-07-01
卷号	5期号:7页码:18
ISSN号	1553-7366
DOI	10.1371/journal.ppat.1000512
通讯作者	Greene, whitney()
英文摘要	The role of actin dynamics in clathrin-mediated endocytosis in mammalian cells is unclear. in this study, we define the role of actin cytoskeleton in kaposi's sarcoma-associated herpesvirus (kshv) entry and trafficking in endothelial cells using an immunofluorescence-based assay to visualize viral capsids and the associated cellular components. in contrast to infectivity or reporter assays, this method does not rely on the expression of any viral and reporter genes, but instead directly tracks the accumulation of individual viral particles at the nuclear membrane as an indicator of successful viral entry and trafficking in cells. inhibitors of endosomal acidification reduced both the percentage of nuclei with viral particles and the total number of viral particles docking at the perinuclear region, indicating endocytosis, rather than plasma membrane fusion, as the primary route for kshv entry into endothelial cells. accordingly, a viral envelope protein was only detected on internalized kshv particles at the early but not late stage of infection. inhibitors of clathrin- but not caveolae/lipid raft-mediated endocytosis blocked kshv entry, indicating that clathrin- mediated endocytosis is the major route of kshv entry into endothelial cells. kshv particles were colocalized not only with markers of early and recycling endosomes, and lysosomes, but also with actin filaments at the early time points of infection. consistent with these observations, transferrin, which enters cells by clathrin- mediated endocytosis, was found to be associated with actin filaments together with early and recycling endosomes, and to a lesser degree, with late endosomes and lysosomes. kshv infection induced dynamic actin cytoskeleton rearrangements. disruption of the actin cytoskeleton and inhibition of regulators of actin nucleation such as rho gtpases and arp2/3 complex profoundly blocked kshv entry and trafficking. together, these results indicate an important role for actin dynamics in the internalization and endosomal sorting/trafficking of kshv and clathrin- mediated endocytosis in endothelial cells.
WOS关键词	CLATHRIN-MEDIATED ENDOCYTOSIS ; POLARIZED EPITHELIAL-CELLS ; MAMMALIAN-CELLS ; SIMPLEX-VIRUS ; CHOLERA-TOXIN ; N-WASP ; LISTERIA-MONOCYTOGENES ; MICROTUBULE DYNAMICS ; MEMBRANE DYNAMICS ; PRIMARY INFECTION
WOS研究方向	Microbiology ; Parasitology ; Virology
WOS类目	Microbiology ; Parasitology ; Virology
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000269224500028
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375531
专题	武汉病毒研究所
通讯作者	Greene, Whitney
作者单位	1.Univ Texas Hlth Sci Ctr San Antonio, Tumor Virol Program, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA 2.Univ Texas Hlth Sci Ctr San Antonio, Dept Pediat, San Antonio, TX 78229 USA 3.Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA 4.Univ Texas Hlth Sci Ctr San Antonio, Canc Therapy & Res Ctr, San Antonio, TX 78229 USA 5.Chinese Acad Sci, Wuhan Inst Virol, Tumor Virol Grp, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Greene, Whitney,Gao, Shou-Jiang. Actin dynamics regulate multiple endosomal steps during kaposi's sarcoma-associated herpesvirus entry and trafficking in endothelial cells[J]. Plos pathogens,2009,5(7):18.
APA	Greene, Whitney,&Gao, Shou-Jiang.(2009).Actin dynamics regulate multiple endosomal steps during kaposi's sarcoma-associated herpesvirus entry and trafficking in endothelial cells.Plos pathogens,5(7),18.
MLA	Greene, Whitney,et al."Actin dynamics regulate multiple endosomal steps during kaposi's sarcoma-associated herpesvirus entry and trafficking in endothelial cells".Plos pathogens 5.7(2009):18.

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