| Essential sequence of influenza b virus hemagglutinin dna to provide protection against lethal homologous viral infection | |
| Wang, Fuyan1,2; Yang, Wen1,2; Fang, Fang2; Chang, Haiyan2; Yu, Ping1; Chen, Ze2,3,4 | |
| 刊名 | Dna and cell biology
 |
| 2008-07-01 | |
| 卷号 | 27期号:7页码:377-385 |
| ISSN号 | 1044-5498 |
| DOI | 10.1089/dna.2007.0706 |
| 通讯作者 | Yu, ping(yuping1953@yahoo.com.cn) |
| 英文摘要 | Hemagglutinin ( ha) is the main surface glycoprotein of influenza b virus. the b/ibaraki/2/85 virus ha gene is 1758 bp in length, including signal peptide sequence, ha1 sequence, and ha2 sequence. we previously proved that b/ibaraki/2/85 ha dna induced immune response and provided effective protection in mice against challenge with homologous virus. in this study, a series of recombinant plasmids encoding truncated ha gene were constructed by pcr. balb/c mice were immunized with the plasmids and challenged with a lethal dose of homologous virus. the essential sequence of ha dna against influenza virus was explored by evaluation of survival rate, lung virus titer, bodyweight change, and serum anti-ha antibody titer of mice. the result showed that serial deletion did not deprive ha dna of its protective ability until 885 nucleotides ( 295 amino acids) at 3'-terminal or 9 nucleotides of the signal peptide sequence at 5'-terminal were deleted. when the signal peptide sequence was kept intact and the 5'-terminal deletion started at the beginning of the ha1 sequence, deletion of 51 nucleotides ( 17 amino acids) made ha dna lose its protective ability. this suggests that the sequence nt94-876 of b/ibaraki/2/85 virus ha dna played an important role in protection against infection. |
| WOS关键词 | NEURAMINIDASE-EXPRESSING DNAS ; MONOCLONAL-ANTIBODIES ; MEMBRANE GLYCOPROTEIN ; ENHANCED PROTECTION ; ANTIGENIC STRUCTURE ; UNITED-STATES ; A-VIRUS ; IMMUNIZATION ; CHALLENGE ; DETERMINANTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology ; Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | MARY ANN LIEBERT INC |
| WOS记录号 | WOS:000258171400005 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375462 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yu, Ping |
| 作者单位 | 1.Cent S Univ, Xiangya Sch Med, Dept Immunol, Changsha 410078, Hunan, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 4.Shanghai Inst Biol Prod, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fuyan,Yang, Wen,Fang, Fang,et al. Essential sequence of influenza b virus hemagglutinin dna to provide protection against lethal homologous viral infection[J]. Dna and cell biology,2008,27(7):377-385. |
| APA | Wang, Fuyan,Yang, Wen,Fang, Fang,Chang, Haiyan,Yu, Ping,&Chen, Ze.(2008).Essential sequence of influenza b virus hemagglutinin dna to provide protection against lethal homologous viral infection.Dna and cell biology,27(7),377-385. |
| MLA | Wang, Fuyan,et al."Essential sequence of influenza b virus hemagglutinin dna to provide protection against lethal homologous viral infection".Dna and cell biology 27.7(2008):377-385. |
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