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	Detection of chromosomally located and plasmid-borne genes on 20 kb dna fragments in parasporal crystals from bacillus thuringiensis
	Sun, Yunjun; Wei, Wei; Ding, Xuezhi; Xia, Liqiu; Yuan, Zhiming
刊名	Archives of microbiology
	2007-10-01
卷号	188期号:4页码:327-332
关键词	Bacillus thuringiensis 20 kb dnas Crystal Chromosome and plasmid Insecticidal activity
ISSN号	0302-8933
DOI	10.1007/s00203-007-0252-7
通讯作者	Xia, liqiu(xialq@hunnu.edu.cn)
英文摘要	The association of 20 kb heterologous dna fragments with the parasporal crystals from native and recombinant bacillus thuringiensis strains was analyzed, respectively. the cry2aa10 gene cloned in plasmid phc39 was transformed into b. thuringiensis subsp. kurstaki strains cry(-)b and hd73, producing recombinant strains cry(-)b(phc39) and hd73(phc39). sds-page and scanning electron microscopy analyses demonstrated that the recombinant cry(-)b(phc39) produced cuboidal crystals of cry2aa10 protoxin, while recombinant hd73(phc39) produced both bipyramidal crystals of cry1ac1 protoxin and cuboidal crystals of cry2aa10 protoxin. bioassay results proved that recombinant hd73(phc39) showed higher insecticidal activity to helicoverpa armigera than cry(-)b(phc39). it was found that 20 kb dna fragments were present in bipyramidal and cuboidal crystals from both native and recombinant strains, and the 20 kb heterologous dnas contained chromosome-specific and resident large plasmid-borne dna fragments, suggesting the 20 kb heterologous dna fragment embodied in crystals came randomly from the bacterial chromosomal and plasmid genome. this was the first investigation devoted exclusively on the origin of 20 kb dna fragments in the parasporal crystals of b. thuringiensis. the data provides a basis for further investigation of the origin of 20 kb dnas in the crystals and the interaction of dna and protoxins.
WOS关键词	CRY-TYPE GENES ; PROTEIN ; IDENTIFICATION ; STRAINS ; SPECIFICITY ; LENGTH
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000249522600004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375419
专题	武汉病毒研究所
通讯作者	Xia, Liqiu
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yunjun,Wei, Wei,Ding, Xuezhi,et al. Detection of chromosomally located and plasmid-borne genes on 20 kb dna fragments in parasporal crystals from bacillus thuringiensis[J]. Archives of microbiology,2007,188(4):327-332.
APA	Sun, Yunjun,Wei, Wei,Ding, Xuezhi,Xia, Liqiu,&Yuan, Zhiming.(2007).Detection of chromosomally located and plasmid-borne genes on 20 kb dna fragments in parasporal crystals from bacillus thuringiensis.Archives of microbiology,188(4),327-332.
MLA	Sun, Yunjun,et al."Detection of chromosomally located and plasmid-borne genes on 20 kb dna fragments in parasporal crystals from bacillus thuringiensis".Archives of microbiology 188.4(2007):327-332.

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