QQ客服 官方微博 反馈留言

	Molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in alcaligenes sp strain nyz215
	Xiao, Yi; Zhang, Jun-He; Liu, Hong; Zhou, Ning-Yi
刊名	Journal of bacteriology
	2007-09-01
卷号	189期号:18页码:6587-6593
ISSN号	0021-9193
DOI	10.1128/jb.00654-07
通讯作者	Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn)
英文摘要	Alcaligenes sp. strain nyz215 was isolated for its ability to grow on ortho-nitrophenol (onp) as the sole source of carbon, nitrogen, and energy and was shown to degrade onp via a catechol ortho-cleavage pathway. a 10,152-bp dna fragment extending from a conserved region of the catechol 1,2-dioxygenase gene was obtained by genome walking. of seven complete open reading frames deduced from this fragment, three (onpabc) have been shown to encode the enzymes involved in the initial reactions of onp catabolism in this strain. onpa, which shares 26% identity with salicylate 1-monooxygenase of pseudomonas stutzeri an10, is an onp 2-monooxygenase (ec 1.14.13.31) which converts onp to catechol in the presence of nadph, with concomitant nitrite release. onpc is a catechol 1,2-dioxygenase catalyzing the oxidation of catechol to cis,cis-muconic acid. onpb exhibits 54% identity with the reductase subunit of vanillate o-demethylase in pseudomonas fluorescens bf13. onpab (but not onpa alone) conferred on the catechol utilizer pseudomonas putida paw340 the ability to grow on onp. this suggests that onpb may also be involved in onp degradation in vivo as an o-benzoquinone reductase converting o-benzoquinone to catechol. this is analogous to the reduction of tetrachlorobenzoquinone to tetrachlorohydroquinone by a tetrachlorobenzoquinone reductase (pcpd, 38% identity with onpb) in the pentachlorophenol degrader sphingobium chlorophenolicum atcc 39723.
WOS关键词	PSEUDOMONAS-SP ; SALICYLATE HYDROXYLASE ; SEQUENCE-ANALYSIS ; P-NITROPHENOL ; TOL PLASMID ; DEGRADATION ; PUTIDA ; BIODEGRADATION ; PATHWAY ; CLONING
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000249384500010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375376
专题	武汉病毒研究所
通讯作者	Zhou, Ning-Yi
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Yi,Zhang, Jun-He,Liu, Hong,et al. Molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in alcaligenes sp strain nyz215[J]. Journal of bacteriology,2007,189(18):6587-6593.
APA	Xiao, Yi,Zhang, Jun-He,Liu, Hong,&Zhou, Ning-Yi.(2007).Molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in alcaligenes sp strain nyz215.Journal of bacteriology,189(18),6587-6593.
MLA	Xiao, Yi,et al."Molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in alcaligenes sp strain nyz215".Journal of bacteriology 189.18(2007):6587-6593.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。