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	The receptor for activated c kinase 1 (rack1) functions in hematopoiesis through jnk activation in chinese mitten crab eriocheir sinensis
	Jia, Zhihao1,3; Wang, Mengqiang1; Wang, Xiudan1,3; Wang, Lingling2; Song, Linsheng2
刊名	Fish & shellfish immunology
	2016-10-01
卷号	57页码:252-261
关键词	Eriocheir sinensis Rack1 Innate immune response Anti-stress Hematopoiesis Jnk
ISSN号	1050-4648
DOI	10.1016/j.fsi.2016.08.036
通讯作者	Song, linsheng(lshsong@dlou.edu.cn)
英文摘要	Receptor for activated c kinase 1 (rack1) is a wd-domain repeating protein which involves in the mediation of various biological processes, including innate immune response. in the present study, a rack1 (designed as esrack1) gene from chinese mitten crab e. sinensis was cloned by rapid amplification of cdna ends (race) technique. the full-length cdna sequence of esrack1 was of 1117 bp with an open reading frame (orf) of 957 bp encoding a polypeptide of 318 amino acids containing seven wd repeats. esrack1 shared 62%-99% similarities with previously identified rack1s in amino acid sequence, and it was clustered with the rack1 from pacifastacus leniusculus in the phylogenetic tree. the mrna transcripts of esrack1 were constitutively expressed in various tissues with the highest expression level in hepatopancreas. the expression of esrack1 mrna in hemocytes were significantly up-regulated post the stimulations with vibrio anguillarum and pichia pastoris. after exposure to cdcl2 and pentachlorophenol, the transcripts of esrack1 in hemocytes were up-regulated at the late phase from 12 h. when esrack1 was knocked down by dsrna based rnai, the total hemocyte counts, newborn hemocytes and phosphorylation of jnk were all significantly decreased. in addition, esrack1 transcription and phosphorylation of jnk were both decreased in hematopoietic tissue post aeromonas hydrophila challenge. all the results suggested that esrack1 was involved in the innate immune response of the crab and participated in the production of new-born hemocytes through activation of jnk. (c) 2016 elsevier ltd. all rights reserved.
WOS关键词	SPOT SYNDROME VIRUS ; PKC-BETA ; LITOPENAEUS-VANNAMEI ; CRASSOSTREA-GIGAS ; IMMUNE-RESPONSES ; PACIFIC OYSTER ; CELL APOPTOSIS ; WHITE SHRIMP ; KAPPA-B ; PROTEIN
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000385327100027
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374874
专题	中国科学院大学
通讯作者	Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jia, Zhihao,Wang, Mengqiang,Wang, Xiudan,et al. The receptor for activated c kinase 1 (rack1) functions in hematopoiesis through jnk activation in chinese mitten crab eriocheir sinensis[J]. Fish & shellfish immunology,2016,57:252-261.
APA	Jia, Zhihao,Wang, Mengqiang,Wang, Xiudan,Wang, Lingling,&Song, Linsheng.(2016).The receptor for activated c kinase 1 (rack1) functions in hematopoiesis through jnk activation in chinese mitten crab eriocheir sinensis.Fish & shellfish immunology,57,252-261.
MLA	Jia, Zhihao,et al."The receptor for activated c kinase 1 (rack1) functions in hematopoiesis through jnk activation in chinese mitten crab eriocheir sinensis".Fish & shellfish immunology 57(2016):252-261.

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