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	Mass spectrometry based strategy for studies of binding sites and structural changes of cisplatin binding to myoglobin
	Liu, Huan1,2,3; Zhang, Ningbo1,3; Cui, Meng1,2; Liu, Zhiqiang2; Liu, Shuying2
刊名	Rapid communications in mass spectrometry
	2016-11-01
卷号	30期号:22页码:2433-2441
ISSN号	0951-4198
DOI	10.1002/rcm.7729
通讯作者	Cui, meng(cuimeng@ciac.ac.cn)
英文摘要	Rationale: it is of great significance to investigate the interaction of the metallodrug cisplatin (cis-[ ptcl2(nh3)(2)]) with myoglobin for understanding of the mechanism of action of cisplatin and the overexpression of myoglobin in tumor cells. methods: the reactions of cisplatin and myoglobin were incubated under different conditions. a mass spectrometry (ms)-based strategy combining full proteolysis and limited proteolysis was developed for comprehensive studies of cisplatin-myoglobin interaction. results: the binding sites of cisplatin on myoglobin were identified as trp14, his64, his81, his113 and his116 using electrospray ionization multiple-stage tandem mass spectrometry (esi-msn) without liquid chromatography (lc) separation. the relative abundances of digested peptides from platinated myoglobin were obviously higher than those from native samples by limited proteolysis. conclusions: an alternative and simple approach was developed to successfully monitor conformational changes of myoglobin induced by cisplatin binding using an esi-ms-based quantification method combined with limited proteolysis. meanwhile, his64 was firstly found to coordinate to platinum, which was likely to affect hydrogen bonds with the oxygen in the heme group. copyright (c) 2016 john wiley & sons, ltd.
WOS关键词	ANTICANCER PLATINUM DRUGS ; PROTEIN-LIGAND COMPLEXES ; EGG-WHITE LYSOZYME ; LIMITED PROTEOLYSIS ; CYTOCHROME-C ; CATION-PI ; TOP-DOWN ; CONFORMATIONAL-CHANGES ; ENDOGENOUS MYOGLOBIN ; SERUM-PROTEINS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
WOS类目	Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000385500900008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374808
专题	中国科学院大学
通讯作者	Cui, Meng
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Synthet Rubber, 5625 Renmin St, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun Ctr Mass Spectrometry, 5625 Renmin St, Changchun 130022, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Liu, Huan,Zhang, Ningbo,Cui, Meng,et al. Mass spectrometry based strategy for studies of binding sites and structural changes of cisplatin binding to myoglobin[J]. Rapid communications in mass spectrometry,2016,30(22):2433-2441.
APA	Liu, Huan,Zhang, Ningbo,Cui, Meng,Liu, Zhiqiang,&Liu, Shuying.(2016).Mass spectrometry based strategy for studies of binding sites and structural changes of cisplatin binding to myoglobin.Rapid communications in mass spectrometry,30(22),2433-2441.
MLA	Liu, Huan,et al."Mass spectrometry based strategy for studies of binding sites and structural changes of cisplatin binding to myoglobin".Rapid communications in mass spectrometry 30.22(2016):2433-2441.

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