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	Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation
	Song, Chunlei1; Yao, Jing2,3; Cao, Chunwei2,3; Liang, Xiaojuan1; Huang, Jiaojiao2,3; Han, Ziqiang2,4; Zhang, Yang2,3; Qin, Guosong2,3; Tao, Cong1; Li, Chengbo5
刊名	Biochemical and biophysical research communications
	2016-10-14
卷号	479期号:2页码:224-230
关键词	Pig Mirna Oocyte maturation Ppar gamma Fatty acid metabolism
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2016.09.046
通讯作者	Wang, yanfang(wangyanfang@caas.cn)
英文摘要	To elucidate the key mirnas and the signalling pathways that are involved in porcine oocyte maturation, we performed a deep sequencing analysis of the mirnas of pig germinal vesicle (gv) oocytes and metaphase ii (mii) oocytes. seven differentially expressed (de) mirnas were identified and the expression levels of mir-21 and mir-27b-3p were further confirmed by qpcr analysis. the target genes of 7 de mirnas were predicted and subjected to pathway analysis. interestingly, fatty acid metabolism and fatty acid biosynthesis were the top two significantly enriched molecular functions during oocyte maturation. heat map, which was built with 7 de mirnas and the enriched the molecular functions, revealed that mir-21, mir-27b-3p, mir-10a-5p and mir-10b-5p were involved in fatty acid metabolism. in particular, the regulatory role of mir-27b-3p on peroxisome proliferator-activated receptor-gamma (ppar gamma) was confirmed by their inversed expression patterns in gv and mii oocytes and luciferase report assays. in addition, we observed that ppar gamma agonist (rosiglitazone) treatment significantly enhanced porcine oocyte maturation rate and early embryo developmental competent. taken together, our results demonstrated that mir-27b and its target, ppar gamma, play the vital roles in pig oocyte maturation through regulating the fatty acid metabolism. these data increased our understanding of the regulatory gene networks in porcine oocyte maturation and development. (c) 2016 elsevier inc. all rights reserved.
WOS关键词	EXPRESSION ; TARGETS ; LIPIDS ; CELLS ; MICE ; PIG
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000385898900017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374806
专题	中国科学院大学
通讯作者	Wang, Yanfang
作者单位	1.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100081, Peoples R China 2.Chinese Acad Sci, State Key Lab Reprod Biol, Inst Zool, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Cunji Med Sch, Beijing, Peoples R China 4.Hebei Univ, Coll Life Sci, Baoding 071000, Peoples R China 5.Rongchang Breeding Co, Binzhou 251900, Shandong, Peoples R China 6.Tsinghua Univ, High Sch, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Song, Chunlei,Yao, Jing,Cao, Chunwei,et al. Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation[J]. Biochemical and biophysical research communications,2016,479(2):224-230.
APA	Song, Chunlei.,Yao, Jing.,Cao, Chunwei.,Liang, Xiaojuan.,Huang, Jiaojiao.,...&Wang, Yanfang.(2016).Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation.Biochemical and biophysical research communications,479(2),224-230.
MLA	Song, Chunlei,et al."Ppar gamma is regulated by mir-27b-3p negatively and plays an important role in porcine oocyte maturation".Biochemical and biophysical research communications 479.2(2016):224-230.

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