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Enhanced dstorm imaging using fluorophores interacting with cucurbituril
Zhang, Min1,2; Gao, Jing1,2; Chen, Junling1,2; Cai, Mingjun1; Jiang, Junguang1; Tian, Zhiyuan3; Wang, Hongda1
刊名Science china-chemistry
2016-07-01
卷号59期号:7页码:848-852
关键词Super-resolution imaging Cucurbituril Photophysical Localization precision
ISSN号1674-7291
DOI10.1007/s11426-015-0535-0
通讯作者Tian, zhiyuan(zytian@ucas.ac.cn) ; Wang, hongda(hdwang@ciac.ac.cn)
英文摘要Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes or proteins as fluorophores. the photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy's localization precision and imaging resolution. fluorophores tamra and atto rho6g, which can interact with macrocyclic host cucurbit[7]uril (cb7) to form host-guest compounds, were found to improve the fluorescence intensity and lifetimes of these dyes. we enhanced the localization precision of direct stochastic optical reconstruction microscopy (dstorm) by introducing cb7 into the imaging buffer, and showed that the number of photons as well as localizations of both tamra and atto rho6g increase over 2 times.
WOS关键词SUPERRESOLUTION FLUORESCENCE MICROSCOPY ; COMPLEXES ; DYES
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
语种英语
出版者SCIENCE PRESS
WOS记录号WOS:000379509500011
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2374555
专题中国科学院大学
通讯作者Tian, Zhiyuan; Wang, Hongda
作者单位1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China
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GB/T 7714
Zhang, Min,Gao, Jing,Chen, Junling,et al. Enhanced dstorm imaging using fluorophores interacting with cucurbituril[J]. Science china-chemistry,2016,59(7):848-852.
APA Zhang, Min.,Gao, Jing.,Chen, Junling.,Cai, Mingjun.,Jiang, Junguang.,...&Wang, Hongda.(2016).Enhanced dstorm imaging using fluorophores interacting with cucurbituril.Science china-chemistry,59(7),848-852.
MLA Zhang, Min,et al."Enhanced dstorm imaging using fluorophores interacting with cucurbituril".Science china-chemistry 59.7(2016):848-852.
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