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	Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy
	Liu, Zheyi1,2; Wang, Fangjun1; Chen, Jin1,2; Zhou, Ye1,2; Zou, Hanfa1
刊名	Journal of chromatography a
	2016-08-26
卷号	1461页码:35-41
关键词	Multi-phosphopeptide enrichment Competitive substitution Pre-coordination Mass spectrometry Phosphoproteomics
ISSN号	0021-9673
DOI	10.1016/j.chroma.2016.07.042
通讯作者	Wang, fangjun(wangfj@dicp.ac.cn)
英文摘要	Although many affinity adsorbents have been developed for phosphopeptides enrichment, high specifically capturing the multi-phosphopeptides is still a big challenge. here, we investigated the mechanism of phosphate ion coordination and substitution on affinity adsorbents surfaces and modulated the selectivity of affinity adsorbents to multi-phosphopeptides based on the different capability of mono and multi-phosphopeptides in competitively substituting the pre-coordinated phosphate ions at strong acidic condition. we demonstrated both the species of pre-coordinated phosphate ions and the substituting conditions played crucial roles in modulating the enrichment selectivity to multi-phosphopeptides, and the pre-coordinated affinity materials with relative more surfaces positive charges exhibited better enrichment efficiency due to the cooperative effect of electrostatic interaction and competitive substitution. finally, an enrichment selectivity of 85% to multi-phosphopeptides was feasibly achieved with 66% improvement in identification numbers for complex protein sample extracted from hepg2 cells. data are available via proteomexchange with identifier pxd004252. (c) 2016 elsevier b.v. all rights reserved.
WOS关键词	PHOSPHORYLATED PEPTIDES ; PROTEIN-PHOSPHORYLATION ; MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; ENRICHMENT ; PHOSPHOPROTEOME ; CHROMATOGRAPHY ; SEPARATION ; NETWORKS ; TIO2
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000381840200005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374412
专题	中国科学院大学
通讯作者	Wang, Fangjun
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zheyi,Wang, Fangjun,Chen, Jin,et al. Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy[J]. Journal of chromatography a,2016,1461:35-41.
APA	Liu, Zheyi,Wang, Fangjun,Chen, Jin,Zhou, Ye,&Zou, Hanfa.(2016).Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy.Journal of chromatography a,1461,35-41.
MLA	Liu, Zheyi,et al."Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy".Journal of chromatography a 1461(2016):35-41.

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