QQ客服 官方微博 反馈留言

	Inhibitory effects of grape skin extract and resveratrol on fatty acid synthase
	Liang,Yan1,2; Tian,Weixi1; Ma,Xiaofeng1
刊名	Bmc complementary and alternative medicine
	2013-12-16
卷号	13期号:1
关键词	Fatty acid synthase β-ketoacyl reductase Inhibitor Grape skin extract Resveratrol Obesity 3?t3-l1 preadipocytes
ISSN号	1472-6882
DOI	10.1186/1472-6882-13-361
通讯作者	Ma,xiaofeng(maxiaofeng@ucas.ac.cn)
英文摘要	Abstractbackgroundgrape skin, a rich source of phytochemicals, has been reported to possess remarkable anti-obesity activity. fatty acid synthase (fas) is a key enzyme catalyzing the synthesis of fatty acid de novo, and has been considered as an anti-obesity target. to elucidate the anti-obesity mechanism of grape skin, we investigated the effects of grape skin extract (gse) and resveratrol, one of the phytochemicals in gse, on fas and fas over-expressed 3?t3-l1 preadipocyte.methodspurified fas was obtained from chicken liver. dried grape skin was extracted by 50% ethanol and partitioned by ethyl acetate. inhibitory effects of gse and resveratrol on fas including fast-binding inhibition, time-dependent inhibition, and enzyme kinetics were determined. inhibitory effects of gse and resveratrol on 3t3-l1 preadipocyte were also measured.resultsgse inhibited the overall reaction and β-ketoacyl reductase (kr) reaction of fas with ic50 values of 4.61?μg/ml and 20.3?μg/ml. for inhibition by resveratrol, the relevant ic50 values were 11.1?μg/ml and 21.9?μg/ml, respectively. and both gse and resveratrol showed time-dependent inhibition for fas, with the kobs values of 0.028?min-1, and 0.040?min-1 respectively. they inhibited the overall reaction of fas competitively with acetyl-coa, noncompetitively with malonyl-coa and in a mixed manner with nadph. moreover, the inhibition on kr domain by resveratrol was time-dependent with kobs value of 0.106?min-1. in 3?t3-l1 preadipocytes, resveratrol reduced lipid accumulation remarkably.conclusionsgse and resveratrol are potent fas inhibitors and they bound reversibly to the kr domain of fas to inhibit the reduction of the saturated acyl groups in fatty acid synthesis. based on the valid data and deliberate analysis, we proposed that gse and resveratrol have great medical potential and officinal value in treating obesity and related diseases.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/1472-6882-13-361
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374321
专题	中国科学院大学
通讯作者	Ma,Xiaofeng
作者单位	1.University of Chinese Academy of Sciences; College of Life Sciences 2.Capital University of Physical Education and Sports; School of Kinesiology and Health
推荐引用方式 GB/T 7714	Liang,Yan,Tian,Weixi,Ma,Xiaofeng. Inhibitory effects of grape skin extract and resveratrol on fatty acid synthase[J]. Bmc complementary and alternative medicine,2013,13(1).
APA	Liang,Yan,Tian,Weixi,&Ma,Xiaofeng.(2013).Inhibitory effects of grape skin extract and resveratrol on fatty acid synthase.Bmc complementary and alternative medicine,13(1).
MLA	Liang,Yan,et al."Inhibitory effects of grape skin extract and resveratrol on fatty acid synthase".Bmc complementary and alternative medicine 13.1(2013).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。