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	Revealing three stages of dna-cisplatin reaction by a solid-state nanopore
	Zhou, Zhi1,2; Hu, Ying1,2; Shan, Xinyan1,2; Li, Wei1,2; Bai, Xuedong1,2,3; Wang, Pengye1,2; Lu, Xinghua1,2,3
刊名	Scientific reports
	2015-07-07
卷号	5页码:9
ISSN号	2045-2322
DOI	10.1038/srep11868
通讯作者	Lu, xinghua(xhlu@iphy.ac.cn)
英文摘要	The dynamic structural behavior in dna due to interaction with cisplatin is essential for the functionality of platinum-based anti-cancer drugs. here we report a novel method to monitor the interaction progress in dna-cisplatin reaction in real time with a solid-state nanopore. the interaction processes are found to be well elucidated by the evolution of the capture rate of dna-cisplatin complex, which is defined as the number of their translocation events through the nanopore in unit time. in the first stage, the capture rate decreases rapidly due to dna discharging as the positive-charged hydrated cisplatin molecules initially bond to the negative-charged dna and form mono-adducts. in the second stage, by forming di-adducts, the capture rate increases as dna molecules are softened, appears as the reduced persistence length of the dna-cisplatin adducts. in the third stage, the capture rate decreases again as a result of dna aggregation. our study demonstrates a new single-molecule tool in exploring dynamic behaviors during drug-dna reactions and may have future application in fast drug screening.
WOS关键词	METAL-COMPLEXES ; SINGLE ; MOLECULES ; ADDUCTS ; CANCER
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000357453300002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374061
专题	物理研究所
通讯作者	Lu, Xinghua
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Phys, Beijing 100190, Peoples R China 3.Collaborat Innovat Ctr Quantum Matter, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Zhi,Hu, Ying,Shan, Xinyan,et al. Revealing three stages of dna-cisplatin reaction by a solid-state nanopore[J]. Scientific reports,2015,5:9.
APA	Zhou, Zhi.,Hu, Ying.,Shan, Xinyan.,Li, Wei.,Bai, Xuedong.,...&Lu, Xinghua.(2015).Revealing three stages of dna-cisplatin reaction by a solid-state nanopore.Scientific reports,5,9.
MLA	Zhou, Zhi,et al."Revealing three stages of dna-cisplatin reaction by a solid-state nanopore".Scientific reports 5(2015):9.

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