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	Characterization of two monoclonal antibodies, 38f10 and 44d11, against the major envelope fusion protein of helicoverpa armigera nucleopolyhedrovirus
	Zou, Zijiao; Liu, Jinliang; Wang, Zhiying; Deng, Fei; Wang, Hualin; Hu, Zhihong; Wang, Manli; Zhang, Tao
刊名	Virologica sinica
	2016-12-01
卷号	31期号:6页码:490-499
关键词	Hearnpv f protein Monoclonal antibody 38f10 44d11 Epitope Neutralizing activity Membrane fusion
ISSN号	1674-0769
DOI	10.1007/s12250-016-3831-4
通讯作者	Wang, manli(wangml@wh.iov.cn) ; Zhang, tao(tzhang@wh.iov.cn)
英文摘要	The envelope fusion protein f of baculoviruses is a class i viral fusion protein which play a significant role during virus entry into insect cells. f is initially synthesized as a precursor (f-0) and then cleaved into a disulfide-linked f-1 and f-2 subunits during the process of protein maturation and secretion. to facilitate further investigation into the structure and function of f protein during virus infection, monoclonal antibodies (mabs) against the f-2 subunit of helicoverpa armigera nucleopolyhedrovirus (hearnpv) (haf) were generated. two kinds of mabs were obtained according to their different recognition epitopes: one kind of mabs, as represented by 38f10, recognizes amino acid (aa) 85 to 123 of f-2 and the other kind, represented by 44d11, recognizes aa 148 to 173 of f-2. western blotand immunofluo rescence assay confirmed that both of the mabs recognized the f protein expressed in hearnpv infected cells, however, only 44d11 could neutralize hearnpv infection. the results further showed that 44d11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the haf protein. the results imply that the monoclonal antibody 44d11 recognizes a region within haf2 that may be involved in the f-mediated membrane fusion process.
WOS关键词	CALIFORNICA MULTICAPSID NUCLEOPOLYHEDROVIRUS ; SINGLE NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; NUCLEAR POLYHEDROSIS-VIRUS ; N-LINKED GLYCOSYLATION ; NEUTRALIZING ANTIBODY ; FUNCTIONAL-ANALYSIS ; GP64 PROTEIN ; F PROTEIN ; IDENTIFICATION ; BACULOVIRUSES
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000393205300005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373474
专题	武汉病毒研究所
通讯作者	Wang, Manli; Zhang, Tao
作者单位	Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Zou, Zijiao,Liu, Jinliang,Wang, Zhiying,et al. Characterization of two monoclonal antibodies, 38f10 and 44d11, against the major envelope fusion protein of helicoverpa armigera nucleopolyhedrovirus[J]. Virologica sinica,2016,31(6):490-499.
APA	Zou, Zijiao.,Liu, Jinliang.,Wang, Zhiying.,Deng, Fei.,Wang, Hualin.,...&Zhang, Tao.(2016).Characterization of two monoclonal antibodies, 38f10 and 44d11, against the major envelope fusion protein of helicoverpa armigera nucleopolyhedrovirus.Virologica sinica,31(6),490-499.
MLA	Zou, Zijiao,et al."Characterization of two monoclonal antibodies, 38f10 and 44d11, against the major envelope fusion protein of helicoverpa armigera nucleopolyhedrovirus".Virologica sinica 31.6(2016):490-499.

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