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	Delivery of vp1 sirna to inhibit the ev71 virus using functionalized silver nanoparticles through ros-mediated signaling pathways
	Li, Yinghua1,3; Lin, Zhengfang1; Xu, Tiantian1; Wang, Changbing1; Zhao, Mingqi1; Xiao, Misi1; Wang, Hanzhong2; Deng, Ning3; Zhu, Bing1,3
刊名	Rsc advances
	2017
卷号	7期号:3页码:1453-1463
ISSN号	2046-2069
DOI	10.1039/c6ra26472g
通讯作者	Deng, ning(dengning123@hotmail.com) ; Zhu, bing(zhubing2016@hotmail.com)
英文摘要	Enterovirus 71 (ev71) is the primary causative agent of hand, foot, and mouth disease (hfmd). there is no effective drug therapy for ev71 at present. small interfering rna (sirna), as a new therapeutic modality, provides a promising antiviral treatment, but it is unable to cross cell membranes. to overcome this limitation, nanotechnology has been proposed to mediate sirna transfection. the antiviral activity of silver nanoparticles (agnps) has attracted increasing attention in recent years and can be employed in biomedical interventions. in this study, a simple method to prepare surface decorated agnps using polyethylenimine (pei) and antiviral sirna has been demonstrated. the development of agnps and pei co-delivery of sirna was designed to be antiviral. mtt assays and tem images showed that pei and sirna-modified agnps (ag@pei@sirna) have remarkable inhibition against ev71 infection and less toxicity to vero cells. the mechanistic investigations revealed that ag@pei@sirna could block ev71 from infecting host cells and prevent dna fragmentation, chromatin condensation and activation of caspase-3. ag@pei@ sirna effectively inhibited the accumulation of reactive oxygen species (ros) by the ev71 virus and activation of akt and p53. taken together, this study demonstrates that ag@pei@ sirna is a novel promising efficient virucide for ev71.
WOS关键词	ENTEROVIRUS 71 VACCINE ; SELENIUM NANOPARTICLES ; IN-VITRO ; DOUBLE-BLIND ; MULTIDRUG-RESISTANCE ; SURFACE DECORATION ; ANTIVIRAL ACTIVITY ; THERAPEUTIC SIRNA ; CELLULAR UPTAKE ; MOUSE MODELS
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000393744400034
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373471
专题	武汉病毒研究所
通讯作者	Deng, Ning; Zhu, Bing
作者单位	1.Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Ctr Lab, Guangzhou 510120, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Beijing, Peoples R China 3.Jinan Univ, Key Lab Mol Immunol & Antibody Engn, Guangdong Prov, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Li, Yinghua,Lin, Zhengfang,Xu, Tiantian,et al. Delivery of vp1 sirna to inhibit the ev71 virus using functionalized silver nanoparticles through ros-mediated signaling pathways[J]. Rsc advances,2017,7(3):1453-1463.
APA	Li, Yinghua.,Lin, Zhengfang.,Xu, Tiantian.,Wang, Changbing.,Zhao, Mingqi.,...&Zhu, Bing.(2017).Delivery of vp1 sirna to inhibit the ev71 virus using functionalized silver nanoparticles through ros-mediated signaling pathways.Rsc advances,7(3),1453-1463.
MLA	Li, Yinghua,et al."Delivery of vp1 sirna to inhibit the ev71 virus using functionalized silver nanoparticles through ros-mediated signaling pathways".Rsc advances 7.3(2017):1453-1463.

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