| Ultrasensitive ebola virus detection based on electroluminescent nanospheres and immunomagnetic separation | |
| Wu, Zhen1; Hu, Jiao1; Zeng, Tao1; Zhang, Zhi-Ling1; Chen, Jianjun2,3; Wong, Gary2,5; Qiu, Xiangguo4; Liu, Wenjun2,5; Gao, George F.2,5,6; Bi, Yuhai2,5,6 | |
| 刊名 | Analytical chemistry
 |
| 2017-02-07 | |
| 卷号 | 89期号:3页码:2039-2048 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b04632 |
| 通讯作者 | Zhang, zhi-ling(zlzhang@whu.edu.cn) ; Bi, yuhai(beeyh@im.ac.cn) |
| 英文摘要 | The 2014-16 ebola virus (ebov) outbreak in west africa has attracted widespread concern. rapid and sensitive detection methods are urgently needed for diagnosis and treatment of the disease. here, we propose a novel method for ebov detection based on efficient amplification of electroluminescent nanospheres (ens) coupled with immunomagnetic separation. uniform ens are made by embedding abundant amounts of cdse/zns quantum dots (qds) into copolymer nanospheres through simple ultrasound. compared to qds, ens can enhance electroluminescence (ecl) signals by approximately 85 fold, achieving a signal to background ratio high enough for ebov detection. the introduction of magnetic nanobeads (mbs) can selectively separate targets from complex samples, simplifying the operation process and saving time. the presence of mbs can amplify ecl by approximately 3 fold, improving detection sensitivity. by integration of ens with mbs, a sensitive electroluminescence biosensor is established for ebov detection. the linear range is 0.02-30 ng/ml with a detection limit of 5.2 pg/ml. this method provides consistent reproducibility, specificity, and anti interference ability and is highly promising in clinical diagnosis applications. |
| WOS关键词 | ENZYME-INDUCED METALLIZATION ; POINT-OF-CARE ; ELECTROGENERATED CHEMILUMINESCENCE ; QUANTUM DOTS ; SENSITIVE DETECTION ; ELECTROCHEMILUMINESCENCE ; NANOCRYSTALS ; IMMUNOSENSOR ; STRATEGY ; ELECTROCHEMISTRY |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000393738300087 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373469 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Zhi-Ling; Bi, Yuhai |
| 作者单位 | 1.Wuhan Univ, State Key Lab Virol, Coll Chem & Mol Sci, Minist Educ,Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Ctr Influenza Res & Early Warning CASCIRE, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 4.Univ Manitoba, Dept Med Microbiol, Publ Hlth Agcy Canada, Natl Microbiol Lab,Special Pathogens Program, Winnipeg, MB R3E 3R2, Canada 5.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 6.Shenzhen Third Peoples Hosp, State Key Discipline Infect Dis, Shenzhen Key Lab Pathogen & Immun, Shenzhen 518112, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Zhen,Hu, Jiao,Zeng, Tao,et al. Ultrasensitive ebola virus detection based on electroluminescent nanospheres and immunomagnetic separation[J]. Analytical chemistry,2017,89(3):2039-2048. |
| APA | Wu, Zhen.,Hu, Jiao.,Zeng, Tao.,Zhang, Zhi-Ling.,Chen, Jianjun.,...&Pang, Dai-Wen.(2017).Ultrasensitive ebola virus detection based on electroluminescent nanospheres and immunomagnetic separation.Analytical chemistry,89(3),2039-2048. |
| MLA | Wu, Zhen,et al."Ultrasensitive ebola virus detection based on electroluminescent nanospheres and immunomagnetic separation".Analytical chemistry 89.3(2017):2039-2048. |
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