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	Orf7 of varicella-zoster virus is required for viral cytoplasmic envelopment in differentiated neuronal cells
	Jiang, Hai-Fei1,2; Wang, Wei3; Jiang, Xuan1; Zeng, Wen-Bo1; Shen, Zhang-Zhou1; Song, Yi-Ge1,2; Yang, Hong1,2; Liu, Xi-Juan1,2; Dong, Xiao1; Zhou, Jing1,2
刊名	Journal of virology
	2017-06-01
卷号	91期号:12页码:17
关键词	Varicella-zoster virus Orf7 Differentiated neural progenitor cells Differentiated sh-sy5y cells Cytoplasmic envelopment
ISSN号	0022-538X
DOI	10.1128/jvi.00127-17
通讯作者	Zhu, hua(zhuhu@njms.rutgers.edu) ; Luo, min-hua(luomh@wh.iov.cn)
英文摘要	Although a varicella-zoster virus (vzv) vaccine has been used for many years, the neuropathy caused by vzv infection is still a major health concern. open reading frame 7 (orf7) of vzv has been recognized as a neurotropic gene in vivo, but its neurovirulent role remains unclear. in the present study, we investigated the effect of orf7 deletion on vzv replication cycle at virus entry, genome replication, gene expression, capsid assembly and cytoplasmic envelopment, and transcellular transmission in differentiated neural progenitor cells (dnpcs) and neuroblastoma sh-sy5y (dsy5y) cells. our results demonstrate that the orf7 protein is a component of the tegument layer of vzv virions. deleting orf7 did not affect viral entry, viral genome replication, or the expression of typical viral genes but clearly impacted cytoplasmic envelopment of vzv capsids, resulting in a dramatic increase of envelope-defective particles and a decrease in intact virions. the defect was more severe in differentiated neuronal cells of dnpcs and dsy5y. orf7 deletion also impaired transmission of orf7-deficient virus among the neuronal cells. these results indicate that orf7 is required for cytoplasmic envelopment of vzv capsids, virus transmission among neuronal cells, and probably the neuropathy induced by vzv infection. importance the neurological damage caused by varicella-zoster virus (vzv) reactivation is commonly manifested as clinical problems. thus, identifying viral neurovirulent genes and characterizing their functions are important for relieving vzv related neurological complications. orf7 has been previously identified as a potential neurotropic gene, but its involvement in vzv replication is unclear. in this study, we found that orf7 is required for vzv cytoplasmic envelopment in differentiated neuronal cells, and the envelopment deficiency caused by orf7 deletion results in poor dissemination of vzv among neuronal cells. these findings imply that orf7 plays a role in neuropathy, highlighting a potential strategy to develop a neurovirulence-attenuated vaccine against chickenpox and herpes zoster and providing a new target for intervention of neuropathy induced by vzv.
WOS关键词	HERPES-SIMPLEX-VIRUS ; TEGUMENT PROTEIN ; PSEUDORABIES VIRUS ; GLYCOPROTEIN-E ; UL51 PROTEIN ; IN-VIVO ; STEM-CELLS ; INFECTION ; REPLICATION ; PATHOGENESIS
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000402169500005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373435
专题	武汉病毒研究所
通讯作者	Zhu, Hua; Luo, Min-Hua
作者单位	1.Chinese Acad Sci, State Key Lab Virol, CAS Ctr Excellence Brain Sci, Wuhan Inst Virol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Xiamen Univ, Sch Publ Hlth, State Key Lab Mol Vaccinol & Mol Diagnost, Xiamen, Peoples R China 4.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan, Peoples R China 5.Univ Oslo, Oslo Univ Hosp, Dept Med Genet, Oslo, Norway 6.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ USA
推荐引用方式 GB/T 7714	Jiang, Hai-Fei,Wang, Wei,Jiang, Xuan,et al. Orf7 of varicella-zoster virus is required for viral cytoplasmic envelopment in differentiated neuronal cells[J]. Journal of virology,2017,91(12):17.
APA	Jiang, Hai-Fei.,Wang, Wei.,Jiang, Xuan.,Zeng, Wen-Bo.,Shen, Zhang-Zhou.,...&Luo, Min-Hua.(2017).Orf7 of varicella-zoster virus is required for viral cytoplasmic envelopment in differentiated neuronal cells.Journal of virology,91(12),17.
MLA	Jiang, Hai-Fei,et al."Orf7 of varicella-zoster virus is required for viral cytoplasmic envelopment in differentiated neuronal cells".Journal of virology 91.12(2017):17.

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