| Infected t98g glioblastoma cells support human cytomegalovirus reactivation from latency | |
| Cheng, Shuang1; Jiang, Xuan1; Yang, Bo1; Wen, Le1; Zhao, Fei1; Zeng, Wen-Bo1; Liu, Xi-Juan1; Dong, Xiao1; Sun, Jin-Yan1; Ming, Ying-Zi2 | |
| 刊名 | Virology
 |
| 2017-10-01 | |
| 卷号 | 510页码:205-215 |
| 关键词 | Human cytomegalovirus Reactivation Latent infection T98g cells Latent cell model of brain origin |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2017.07.023 |
| 通讯作者 | Fortunato, elizabeth(lfort@uidaho.edu) ; Luo, min-hua(luomh@wh.iov.cn) |
| 英文摘要 | T98g cells have been shown to support long-term human cytomegalovirus (hcmv) genome maintenance without infectious virus release. however, it remains unclear whether these viral genomes could be reactivated. to address this question, a recombinant hcmv (rhcmv) containing a gfp gene was used to infect t98g cells, and the infected cells absent of infectious virus production were designated t98g-lrv. upon dibutyryl camp plus ibmx (camp/ibmx) treatment, a serial of phenomena were observed, including gfp signal increase, viral genome replication, lytic genes expression and infectious viruses release, indicating the reactivation of hcmv in t98g-lrv cells from a latent status. mechanistically, hcmv reactivation in the t98g-lrv cells induced by camp/ibmx was associated with the pka-creb signaling pathway. these results demonstrate that hcmv was latent in t98g-lrv cells and could be reactivated. the t98g-lrv cells represent an effective model for investigating the mechanisms of hcmv reactivation from latency in the context of neural cells. |
| WOS关键词 | EMBRYONAL NTERA2 CELLS ; BRAIN-DERIVED CELLS ; GENE-EXPRESSION ; CYCLIC-AMP ; CD14(+) MONOCYTES ; VIRUS ; REPLICATION ; DIFFERENTIATION ; PROTEIN ; LINE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000408400100023 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373377 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fortunato, Elizabeth; Luo, Min-Hua |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Cent S Univ, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China 3.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07101 USA 4.Univ Oslo, Oslo Univ Hosp, Dept Med Genet, N-0316 Oslo, Norway 5.Howard Univ, Coll Med, Dept Microbiol, Washington, DC 20059 USA 6.Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA 7.Univ Idaho, Ctr Reprod Biol, Moscow, ID 83844 USA 8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Shuang,Jiang, Xuan,Yang, Bo,et al. Infected t98g glioblastoma cells support human cytomegalovirus reactivation from latency[J]. Virology,2017,510:205-215. |
| APA | Cheng, Shuang.,Jiang, Xuan.,Yang, Bo.,Wen, Le.,Zhao, Fei.,...&Luo, Min-Hua.(2017).Infected t98g glioblastoma cells support human cytomegalovirus reactivation from latency.Virology,510,205-215. |
| MLA | Cheng, Shuang,et al."Infected t98g glioblastoma cells support human cytomegalovirus reactivation from latency".Virology 510(2017):205-215. |
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