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	Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis
	Zhang, Z. -T.1,2; Wang, D. -B.2; Li, C. -Y.3; Deng, J. -Y.4; Zhang, J. -B.1; Bi, L. -J.2; Zhang, X. -E.2
刊名	Journal of applied microbiology
	2018
卷号	124期号:1页码:286-293
关键词	Drug resistance Drug susceptibility testing Microbial sensor Mycobacterium tuberculosis Oxygen electrode
ISSN号	1364-5072
DOI	10.1111/jam.13617
通讯作者	Zhang, x. -e.(zhangxe@ibp.ac.cn)
英文摘要	Aimsdrug susceptibility testing (dst) of clinical isolates of mycobacterium tuberculosis is critical in treating tuberculosis. we demonstrate the possibility of using a microbial sensor to perform dst of m. tuberculosis and shorten the time required for dst. methods and resultsthe sensor is made of an oxygen electrode with m. tuberculosis cells attached to its surface. this sensor monitors the residual oxygen consumption of m. tuberculosis cells after treatment with anti-tb drugs with glycerine as a carbon source. in principle, after drug pretreatment for 4-5days, the response differences between the sensors made of drug-sensitive isolates are distinguishable from the sensors made of drug-resistant isolates. the susceptibility of the m. tuberculosis h37ra strain, its mutants and 35 clinical isolates to six common anti-tb drugs: rifampicin, isoniazid, streptomycin, ethambutol, levofloxacin and para-aminosalicylic acid were tested using the proposed method. the results agreed well with the gold standard method (lj) and were determined in significantly less time. the whole procedure takes approximately 11days and therefore has the potential to inform clinical decisions. conclusionsto our knowledge, this is the first study that demonstrates the possible application of a dissolved oxygen electrode-based microbial sensor in m. tuberculosis drug resistance testing. this study used the microbial sensor to perform dst of m. tuberculosis and shorten the time required for dst. significance and impact of the studythe overall detection result of the microbial sensor agreed well with that of the conventional lj proportion method and takes less time than the existing phenotypic methods. in future studies, we will build an o-2 electrode array microbial sensor reactor to enable a high-throughput drug resistance analysis.
WOS关键词	POINT-OF-CARE ; MTBDRPLUS ASSAY ; RESISTANCE ; RIFAMPIN ; TECHNOLOGIES ; DIAGNOSTICS ; ACID
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000418453800026
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373357
专题	武汉病毒研究所
通讯作者	Zhang, X. -E.
作者单位	1.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agromicrobiol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing, Peoples R China 3.Capital Med Univ, Beijing Chest Hosp, Beijing, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Z. -T.,Wang, D. -B.,Li, C. -Y.,et al. Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis[J]. Journal of applied microbiology,2018,124(1):286-293.
APA	Zhang, Z. -T..,Wang, D. -B..,Li, C. -Y..,Deng, J. -Y..,Zhang, J. -B..,...&Zhang, X. -E..(2018).Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis.Journal of applied microbiology,124(1),286-293.
MLA	Zhang, Z. -T.,et al."Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis".Journal of applied microbiology 124.1(2018):286-293.

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