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	Prevalence and genetic diversity analysis of human coronaviruses among cross-border children
	Liu, Peilin1,2,3; Shi, Lei2,3; Zhang, Wei4; He, Jianan2,3; Liu, Chunxiao2,3; Zhao, Chunzhong2,3; Kong, Siu Kai5; Loo, Jacky Fong Chuen5,6; Gu, Dayong2,3,7; Hu, Longfei2,3,7
刊名	Virology journal
	2017-11-22
卷号	14页码:8
关键词	Human coronaviruses Cross-border children Molecular epidemiology Phylogenetic analysis Genetic diversity
ISSN号	1743-422X
DOI	10.1186/s12985-017-0896-0
通讯作者	Loo, jacky fong chuen(jackyfcloo@cuhk.edu.hk) ; Gu, dayong(wanhood@163.com) ; Hu, longfei(18307550009@139.com)
英文摘要	Background: more than a decade after the outbreak of human coronaviruses (hcovs) sars in guangdong province and hong kong sar of china in 2002, there is still no reoccurrence, but the evolution and recombination of the coronaviruses in this region are still unknown. therefore, surveillance on the prevalence and the virus variation of hcovs circulation in this region is conducted. methods: a total of 3298 nasopharyngeal swabs samples were collected from cross-border children (<6 years, crossing border between southern china and hong kong sar) showing symptoms of respiratory tract infection, such as fever (body temperature >37.5 degrees c), from 2014 may to 2015 dec. viral nucleic acids were analyzed and sequenced to study the prevalence and genetic diversity of the four human coronaviruses. the statistical significance of the data was evaluated with fisher chi-square test. results: 78 (2.37%; 95% ci 1.8-2.8%) out of 3298 nasopharyngeal swabs specimens were found to be positive for oc43 (36;1.09%), hku1 (34; 1.03%), nl63 (6; 0.18%) and 229e (2;0.01%). none of sars or mers was detected. the hcovs predominant circulating season was in transition of winter to spring, especially january and february and nl63 detected only in summer and fall. complex population with an abundant genetic diversity of coronaviruses was circulating and they shared homology with the published strains (99-100%). besides, phylogenetic evolutionary analysis indicated that oc43 coronaviruses were clustered into three clades (b,d,e), hku1 clustered into two clades(a,b) and nl63 clustered into two clades(a,b). moreover, several novel mutations including nucleotides substitution and the insertion of spike of the glycoprotein on the viral surface were discovered. conclusions: the detection rate and epidemic trend of coronaviruses were stable and no obvious fluctuations were found. the detected coronaviruses shared a conserved gene sequences in s and rdrp. however, mutants of the epidemic strains were detected, suggesting continuous monitoring of the human coronaviruses is in need among cross-border children, who are more likely to get infected and transmit the viruses across the border easily, in addition to the general public.
WOS关键词	GENOMIC ANALYSIS ; OC43 ; NL63 ; INFECTIONS ; HKU1 ; RECOMBINATION ; CIRCULATION ; GENOTYPE ; REVEALS ; DOMAIN
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000416003500001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373304
专题	武汉病毒研究所
通讯作者	Loo, Jacky Fong Chuen; Gu, Dayong; Hu, Longfei
作者单位	1.Sun Yat Sen Univ, Sch Publ Hlth, Dept Hlth Inspect & Quarantine, Guangzhou 510275, Guangdong, Peoples R China 2.Shenzhen Int Travel Hlth Care Ctr, Cent Lab Hlth Quarantine, Shenzhen 518033, Peoples R China 3.Shenzhen Acad Inspect & Quarantine, Shenzhen Entry Exit Inspect & Quarantine Bur, Shenzhen 518033, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 5.Chinese Univ Hong Kong, Sch Life Sci, Biochem Programme, Hong Kong, Hong Kong, Peoples R China 6.Chinese Univ Hong Kong, Dept Biomed Engn, Hong Kong, Hong Kong, Peoples R China 7.Shenzhen Acad Inspect & Quarantine, Shenzhen 518010, Peoples R China
推荐引用方式 GB/T 7714	Liu, Peilin,Shi, Lei,Zhang, Wei,et al. Prevalence and genetic diversity analysis of human coronaviruses among cross-border children[J]. Virology journal,2017,14:8.
APA	Liu, Peilin.,Shi, Lei.,Zhang, Wei.,He, Jianan.,Liu, Chunxiao.,...&Hu, Longfei.(2017).Prevalence and genetic diversity analysis of human coronaviruses among cross-border children.Virology journal,14,8.
MLA	Liu, Peilin,et al."Prevalence and genetic diversity analysis of human coronaviruses among cross-border children".Virology journal 14(2017):8.

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