| Absolute copy numbers of -actin proteins collected from 10,000 single cells | |
| Fan, Beiyuan1,2; Li, Xiufeng1,2; Liu, Lixing1,2; Chen, Deyong1,2; Cao, Shanshan3; Men, Dong3; Wang, Junbo1,2; Chen, Jian1,2 | |
| 刊名 | Micromachines
 |
| 2018-05-01 | |
| 卷号 | 9期号:5页码:9 |
| 关键词 | Microfluidics Single-cell analysis Polymeric microfluidic flow cytometry Single-cell protein quantification |
| ISSN号 | 2072-666X |
| DOI | 10.3390/mi9050254 |
| 通讯作者 | Wang, junbo(jbwang@mail.ie.ac.cn) ; Chen, jian(chenjian@mail.ie.ac.cn) |
| 英文摘要 | Semi-quantitative studies have located varied expressions of -actin proteins at the population level, questioning their roles as internal controls in western blots, while the absolute copy numbers of -actins at the single-cell level are missing. in this study, a polymeric microfluidic flow cytometry was used for single-cell analysis, and the absolute copy numbers of single-cell -actin proteins were quantified as 9.9 +/- 4.6 x 10(5), 6.8 +/- 4.0 x 10(5) and 11.0 +/- 5.5 x 10(5) per cell for a549 (n(cell) = 14,754), hep g2 (n(cell) = 36,949), and hela (n(cell) = 24,383), respectively. high coefficients of variation (similar to 50%) and high quartile coefficients of dispersion (similar to 30%) were located, indicating significant variations of -actin proteins within the same cell type. low p values (<<0.01) and high classification rates based on neural network (similar to 70%) were quantified among a549, hep g2 and hela cells, suggesting expression differences of -actin proteins among three cell types. in summary, the results reported here indicate significant variations of -actin proteins within the same cell type from cell to cell, and significant expression differences of -actin proteins among different cell types, strongly questioning the properties of using -actin proteins as internal controls in western blots. |
| WOS关键词 | BETA-ACTIN ; MASS CYTOMETRY ; HOUSEKEEPING PROTEINS ; INTERNAL CONTROL ; EXPRESSION ; MICE ; TUBULIN ; IMMUNE ; RNA |
| WOS研究方向 | Science & Technology - Other Topics ; Instruments & Instrumentation |
| WOS类目 | Nanoscience & Nanotechnology ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000435195500058 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373259 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Junbo; Chen, Jian |
| 作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Beiyuan,Li, Xiufeng,Liu, Lixing,et al. Absolute copy numbers of -actin proteins collected from 10,000 single cells[J]. Micromachines,2018,9(5):9. |
| APA | Fan, Beiyuan.,Li, Xiufeng.,Liu, Lixing.,Chen, Deyong.,Cao, Shanshan.,...&Chen, Jian.(2018).Absolute copy numbers of -actin proteins collected from 10,000 single cells.Micromachines,9(5),9. |
| MLA | Fan, Beiyuan,et al."Absolute copy numbers of -actin proteins collected from 10,000 single cells".Micromachines 9.5(2018):9. |
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