Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment | |
Fang, Junnan1,2; Jing, Xuping3,4; Lu, Guoliang3,4; Xu, Yi1,2; Gong, Peng1,2,3 | |
刊名 | Acs infectious diseases |
2019-02-01 | |
卷号 | 5期号:2页码:177-183 |
关键词 | Flavivirus Zika virus Ns3 helicase Atp hydrolysis Reactant water |
ISSN号 | 2373-8227 |
DOI | 10.1021/acsinfecdis.8b00214 |
通讯作者 | Xu, yi(xuyi70@163.com) ; Gong, peng(gongpeng@wh.iov.cn) |
英文摘要 | Zika virus (zikv), a positive-strand rna virus belonging to the flavivirus genus, has become an urgent public health concern since recent outbreaks worldwide. its genome replication is facilitated by the viral ns3 protein bearing helicase function. the ns3 helicase uses energy derived from adenosine triphosphate (atp) hydrolysis to unwind rna duplexed regions. structural studies of the flavivirus ns3 helicases have suggested a conserved mechanism of atp hydrolysis. however, the process of the reactant water replenishment, a key part of the hydrolysis cycle, remains elusive. here, we report two high-resolution crystal structures of zikv ns3 helicase in complex with adenosine diphosphate (adp) and mn2+, one with the reactant water already loaded as previously observed and the other with the water molecule still in a loading state. these data suggest that the reactant water replenishment can occur between the release of phosphate and the release of adp and improves the structural basis of the ns3 atp hydrolysis cycle. |
WOS关键词 | STRUCTURAL BASIS ; RNA ; MECHANISM ; TRANSLOCATION ; SOFTWARE ; INSIGHTS |
WOS研究方向 | Pharmacology & Pharmacy ; Infectious Diseases |
WOS类目 | Chemistry, Medicinal ; Infectious Diseases |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000458937100003 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2373172 |
专题 | 武汉病毒研究所 |
通讯作者 | Xu, Yi; Gong, Peng |
作者单位 | 1.Guangzhou Women & Childrens Med Ctr, Guangzhou Inst Pediat, Joint Ctr Translat Precis Med, 318 Renminzhonglu, Guangzhou 510623, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Ctr Translat Precis Med, 44 Xiao Hong Shan, Wuhan 430071, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, 44 Xiao Hong Shan, Wuhan 430071, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Fang, Junnan,Jing, Xuping,Lu, Guoliang,et al. Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment[J]. Acs infectious diseases,2019,5(2):177-183. |
APA | Fang, Junnan,Jing, Xuping,Lu, Guoliang,Xu, Yi,&Gong, Peng.(2019).Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment.Acs infectious diseases,5(2),177-183. |
MLA | Fang, Junnan,et al."Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment".Acs infectious diseases 5.2(2019):177-183. |
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