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	Quality control of serum and plasma by quantification of (4e, 14z)-sphingadienine-c18-1-phosphate uncovers common preanalytical errors during handling of whole blood
	Liu, Xinyu1,2; Hoene, Miriam3; Yin, Peiyuan1; Fritsche, Louise4,5; Plomgaard, Peter6,7,8,9; Hansen, Jakob S.7,8,9; Nakas, Christos T.10,11; Niess, Andreas M.12; Hudemann, Jens12; Haap, Michael13
刊名	Clinical chemistry
	2018-05-01
卷号	64期号:5页码:810-819
ISSN号	0009-9147
DOI	10.1373/clinchem.2017.277905
通讯作者	Xu, guowang(xugw@dicp.ac.cn) ; Lehmann, rainer(rainer.lehmann@med.uni-tuebingen.de)
英文摘要	Background: nonadherence to standard operating procedures (sops) during handling and processing of whole blood is one of the most frequent causes affecting the quality of serum and plasma. yet, the quality of blood samples is of the utmost importance for reliable, conclusive research findings, valid diagnostics, and appropriate therapeutic decisions. methods: uhplc-ms-driven nontargeted metabolomics was applied to identify biomarkers that reflected time to processing of blood samples, and a targeted uhplc-ms analysis was used to quantify and validate these biomarkers. results: we found that (4e, 14z)-sphingadienine-c181-phosphate (s1p-d18: 2) was suitable for the reliable assessment of the pronounced changes in the quality of serum and plasma caused by errors in the phase between collection and centrifugation of whole blood samples. we rigorously validated s1p-d18: 2, which included the use of practicality tests on >1400 randomly selected serum and plasma samples that were originally collected during single-and multicenter trials and then stored in 11 biobanks in 3 countries. neither life-threatening disease states nor strenuous metabolic challenges (i.e., highintensity exercise) affected the concentration of s1p-d18: 2. cutoff values for sample assessment were defined (plasma, <= 0.085 mu g/ml; serum, <= 0.154 mu g/ml). conclusions: unbiased valid monitoring to check for adherence to sop-dictated time for processing to plasma or serum and/or time to storage of whole blood at 4 degrees c is now feasible. this novel quality assessment step could enable scientists to uncover common preanalytical errors, allowing for identification of serum and plasma samples that should be excluded from certain investigations. it should also allow control of samples before long-term storage in biobanks. (c) 2018 american association for clinical chemistry
WOS关键词	SPHINGOSINE 1-PHOSPHATE ; UK BIOBANK ; WASTE
WOS研究方向	Medical Laboratory Technology
WOS类目	Medical Laboratory Technology
语种	英语
出版者	AMER ASSOC CLINICAL CHEMISTRY
WOS记录号	WOS:000448290000010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373149
专题	大连化学物理研究所
通讯作者	Xu, Guowang; Lehmann, Rainer
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Hosp Tubingen, Cent Lab, Div Clin Chem & Pathobiochem, Hoppe Seyler Str 3, D-72076 Tubingen, Germany 4.Univ Tubingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, Clin Chem Lab,German Ctr Diabet Res DZD,Core Faci, Tubingen, Germany 5.German Ctr Diabet Res DZD, Tubingen, Germany 6.Rigshosp, Dept Clin Biochem, Copenhagen, Denmark 7.Rigshosp, Ctr Inflammat & Metab, Copenhagen, Denmark 8.Rigshosp, Ctr Phys Act Res, Dept Infect Dis, Copenhagen, Denmark 9.Rigshosp, Copenhagen Muscle Res Ctr, Copenhagen, Denmark 10.Univ Bern, Bern Univ Hosp, Inselspital, Ctr Lab Med,Univ Inst Clin Chem, Bern, Switzerland
推荐引用方式 GB/T 7714	Liu, Xinyu,Hoene, Miriam,Yin, Peiyuan,et al. Quality control of serum and plasma by quantification of (4e, 14z)-sphingadienine-c18-1-phosphate uncovers common preanalytical errors during handling of whole blood[J]. Clinical chemistry,2018,64(5):810-819.
APA	Liu, Xinyu.,Hoene, Miriam.,Yin, Peiyuan.,Fritsche, Louise.,Plomgaard, Peter.,...&Lehmann, Rainer.(2018).Quality control of serum and plasma by quantification of (4e, 14z)-sphingadienine-c18-1-phosphate uncovers common preanalytical errors during handling of whole blood.Clinical chemistry,64(5),810-819.
MLA	Liu, Xinyu,et al."Quality control of serum and plasma by quantification of (4e, 14z)-sphingadienine-c18-1-phosphate uncovers common preanalytical errors during handling of whole blood".Clinical chemistry 64.5(2018):810-819.

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