Rapid quantitative fluorescence detection of copper ions with disposable microcapsule arrays utilizing functional nucleic acid strategy | |
He, Enqi1,4; Cai, Liangyuan2; Zheng, Fengyi3; Zhou, Qianyu2; Guo, Dan1; Zhou, Yinglin2; Zhang, Xinxiang2; Li, Zhihong3,4 | |
刊名 | Scientific reports |
2019-01-10 | |
卷号 | 9页码:8 |
ISSN号 | 2045-2322 |
DOI | 10.1038/s41598-018-36842-x |
通讯作者 | Zhou, yinglin(zhouyl@pku.edu.cn) ; Zhang, xinxiang(zxx@pku.edu.cn) ; Li, zhihong(zhhli@pku.edu.cn) |
英文摘要 | In this work, an economical and easy-to-use microcapsule array fabricated by ice printing technique has been realized for ultrasensitive fluorescence quantification of copper ions employing functional nucleic acid strategy. with ice printing, the detection reagents are sealed by polystyrene (ps) film isolation and photopolymer, which guarantees a stable and contamination-free environment for functional nucleic acid reaction. our microcapsule arrays have shown long-term stability (20 days) under -20 degrees c storage in frozen form before use. during the cu2+ on-site detection, 1 mu l sample is simply injected into the thawy microcapsule by a microliter syringe under room temperature, and after 20 minutes the fluorescence result can be obtained by an led transilluminator. this method can realize the detection limit to 100 nm (100 fmol/mu l) with high specificity. |
WOS关键词 | CATALYTIC BEACON SENSOR ; ON-A-CHIP ; HIGH-SENSITIVITY ; DNA ; MERCURY ; ENZYME |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
语种 | 英语 |
出版者 | NATURE PUBLISHING GROUP |
WOS记录号 | WOS:000455352400019 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2372878 |
专题 | 大连化学物理研究所 |
通讯作者 | Zhou, Yinglin; Zhang, Xinxiang; Li, Zhihong |
作者单位 | 1.Tsinghua Univ, Dept Mech Engn, State Key Lab Tribol, Beijing 100084, Peoples R China 2.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,BNLMS, Beijing 100871, Peoples R China 3.Peking Univ, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China 4.Tsinghua Univ, Ctr Nano & Micro Mech, Beijing 100084, Peoples R China |
推荐引用方式 GB/T 7714 | He, Enqi,Cai, Liangyuan,Zheng, Fengyi,et al. Rapid quantitative fluorescence detection of copper ions with disposable microcapsule arrays utilizing functional nucleic acid strategy[J]. Scientific reports,2019,9:8. |
APA | He, Enqi.,Cai, Liangyuan.,Zheng, Fengyi.,Zhou, Qianyu.,Guo, Dan.,...&Li, Zhihong.(2019).Rapid quantitative fluorescence detection of copper ions with disposable microcapsule arrays utilizing functional nucleic acid strategy.Scientific reports,9,8. |
MLA | He, Enqi,et al."Rapid quantitative fluorescence detection of copper ions with disposable microcapsule arrays utilizing functional nucleic acid strategy".Scientific reports 9(2019):8. |
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