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	Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants
	Du, Nana1,2; Si, Wei1; Liu, Yanfang1; Guo, Zhimou1; Zhou, Weijia1,2; Zhou, Han1; Liang, Xinmiao1; Wang, Chaoran1
刊名	Journal of chromatography b-analytical technologies in the biomedical and life sciences
	2019-01-15
卷号	1105页码:148-155
关键词	Alkaloid enrichment Electrostatic repulsion Reversed-phase/strong anion-exchange Scopolia tangutica Mixed-mode stationary phase Class separation
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2018.12.024
通讯作者	Liu, yanfang(liuyanfang@dicp.ac.cn) ; Liang, xinmiao(liangxm@dicp.ac.cn)
英文摘要	Since the content of alkaloids is usually low in plants and they are easily co-eluted with other constituents, enrichment of alkaloids is essential in the discovery of bioactive lead compounds from natural products. in this paper, an easy spe enrichment method was developed in a buffer-free solvent system based on electrostatic repulsion mechanism. the feasibility of the new method was verified by successful enrichment of alkaloids from scopolia tangutica (s. tangutica) with an optimized eluting condition. then this developed method was applied to other representative plants in different families, including przewalskia tangutica and peganum harmala l, lycoris radiata and menispermum dauricum dc, which enlarged the scope of applicability. additionally, the new spe procedure avoided possible structural change destruction caused by ph change. simple solvent system, including formic acid (fa) and methanol, would benefit subsequent mass analysis, quantity determination and bioactivity screening, and so on.
WOS关键词	LIQUID-CHROMATOGRAPHY ; ACONITUM ALKALOIDS ; ALZHEIMERS-DISEASE ; PHASE ; EXTRACTION ; SEPARATION ; SURFACE ; COLUMN
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000456893100017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372875
专题	大连化学物理研究所
通讯作者	Liu, Yanfang; Liang, Xinmiao
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Du, Nana,Si, Wei,Liu, Yanfang,et al. Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2019,1105:148-155.
APA	Du, Nana.,Si, Wei.,Liu, Yanfang.,Guo, Zhimou.,Zhou, Weijia.,...&Wang, Chaoran.(2019).Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants.Journal of chromatography b-analytical technologies in the biomedical and life sciences,1105,148-155.
MLA	Du, Nana,et al."Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants".Journal of chromatography b-analytical technologies in the biomedical and life sciences 1105(2019):148-155.

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